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Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study

BACKGROUND: Currently, obesity and its comorbidities have become a serious threat to human health necessitating urgent development of safe and effective therapy for their management. MATERIALS AND METHODS: In this research, a novel polyherbal formulation (F2) was prepared by mixing specific proporti...

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Autores principales: Pandeya, Prakash Raj, Lamichhane, Ramakanta, Lee, Kyung-Hee, Lamichhane, Gopal, Kim, Se-Gun, Jung, Hyun-Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884115/
https://www.ncbi.nlm.nih.gov/pubmed/33628322
http://dx.doi.org/10.1155/2021/8854915
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author Pandeya, Prakash Raj
Lamichhane, Ramakanta
Lee, Kyung-Hee
Lamichhane, Gopal
Kim, Se-Gun
Jung, Hyun-Ju
author_facet Pandeya, Prakash Raj
Lamichhane, Ramakanta
Lee, Kyung-Hee
Lamichhane, Gopal
Kim, Se-Gun
Jung, Hyun-Ju
author_sort Pandeya, Prakash Raj
collection PubMed
description BACKGROUND: Currently, obesity and its comorbidities have become a serious threat to human health necessitating urgent development of safe and effective therapy for their management. MATERIALS AND METHODS: In this research, a novel polyherbal formulation (F2) was prepared by mixing specific proportions of royal jelly and lemon juice with ethanol extracts of Orostachys japonicus, Rhus verniciflua, and Geranium thunbergii. The antioxidant activity was assessed using DPPH and ABTS assay methods. The antiobesity potential of the F2 was assessed in vitro using 3T3-L1 fibroblast and in vivo using a high-fat diet (HFD) fed C57BL/6J mice model. F2 was administered in mice at the dose of 23 mg/kg and 46 mg/kg, twice daily by oral gavage. A well-accepted antiobesity agent, Garcinia cambogia (GC), at 200 mg/kg was used as a positive control. RESULTS: F2 was observed to exhibit synergistic antiadipogenic activity in 3T3-L1 cells. This inhibition was reinforced by the downregulation of specific adipogenic transcription factors. Furthermore, F2 was also found to reduce mice body weight gain, food efficiency ratio, fasting blood glucose level, fat deposition into the liver, and mass of white adipose tissue. F2 also played a role in the excretion of fat consumed by the mice. For most of the assays performed, the F2 (46 mg/kg) was comparable to the positive control GC (200 mg/kg). In addition, potential and synergistic antioxidant activity was observed on F2. CONCLUSION: The results revealed that the formulation F2 exhibited potential antiobesity activity through the inhibition of adipocyte differentiation, dietary fat absorption, and reduction of free fatty acids deposition in tissues.
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spelling pubmed-78841152021-02-23 Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study Pandeya, Prakash Raj Lamichhane, Ramakanta Lee, Kyung-Hee Lamichhane, Gopal Kim, Se-Gun Jung, Hyun-Ju Evid Based Complement Alternat Med Research Article BACKGROUND: Currently, obesity and its comorbidities have become a serious threat to human health necessitating urgent development of safe and effective therapy for their management. MATERIALS AND METHODS: In this research, a novel polyherbal formulation (F2) was prepared by mixing specific proportions of royal jelly and lemon juice with ethanol extracts of Orostachys japonicus, Rhus verniciflua, and Geranium thunbergii. The antioxidant activity was assessed using DPPH and ABTS assay methods. The antiobesity potential of the F2 was assessed in vitro using 3T3-L1 fibroblast and in vivo using a high-fat diet (HFD) fed C57BL/6J mice model. F2 was administered in mice at the dose of 23 mg/kg and 46 mg/kg, twice daily by oral gavage. A well-accepted antiobesity agent, Garcinia cambogia (GC), at 200 mg/kg was used as a positive control. RESULTS: F2 was observed to exhibit synergistic antiadipogenic activity in 3T3-L1 cells. This inhibition was reinforced by the downregulation of specific adipogenic transcription factors. Furthermore, F2 was also found to reduce mice body weight gain, food efficiency ratio, fasting blood glucose level, fat deposition into the liver, and mass of white adipose tissue. F2 also played a role in the excretion of fat consumed by the mice. For most of the assays performed, the F2 (46 mg/kg) was comparable to the positive control GC (200 mg/kg). In addition, potential and synergistic antioxidant activity was observed on F2. CONCLUSION: The results revealed that the formulation F2 exhibited potential antiobesity activity through the inhibition of adipocyte differentiation, dietary fat absorption, and reduction of free fatty acids deposition in tissues. Hindawi 2021-02-08 /pmc/articles/PMC7884115/ /pubmed/33628322 http://dx.doi.org/10.1155/2021/8854915 Text en Copyright © 2021 Prakash Raj Pandeya et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pandeya, Prakash Raj
Lamichhane, Ramakanta
Lee, Kyung-Hee
Lamichhane, Gopal
Kim, Se-Gun
Jung, Hyun-Ju
Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study
title Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study
title_full Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study
title_fullStr Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study
title_full_unstemmed Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study
title_short Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study
title_sort efficacy of a novel herbal formulation (f2) on the management of obesity: in vitro and in vivo study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884115/
https://www.ncbi.nlm.nih.gov/pubmed/33628322
http://dx.doi.org/10.1155/2021/8854915
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