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First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India

Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever (SA-MCF), a highly fatal disease syndrome that predominantly affects susceptible hosts of the order Artiodactyla. In this study, an in-depth clinico-molecular investigation of SA-MCF disease in a morbid...

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Autores principales: Kumar, Naveen, Sood, Richa, Pateriya, Atul K., Venkatesakumar, E., Ramprabhu, R., Dixit, Roma, Bhatia, Sandeep, Singh, Vijendra Pal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884311/
https://www.ncbi.nlm.nih.gov/pubmed/33604365
http://dx.doi.org/10.3389/fvets.2021.610178
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author Kumar, Naveen
Sood, Richa
Pateriya, Atul K.
Venkatesakumar, E.
Ramprabhu, R.
Dixit, Roma
Bhatia, Sandeep
Singh, Vijendra Pal
author_facet Kumar, Naveen
Sood, Richa
Pateriya, Atul K.
Venkatesakumar, E.
Ramprabhu, R.
Dixit, Roma
Bhatia, Sandeep
Singh, Vijendra Pal
author_sort Kumar, Naveen
collection PubMed
description Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever (SA-MCF), a highly fatal disease syndrome that predominantly affects susceptible hosts of the order Artiodactyla. In this study, an in-depth clinico-molecular investigation of SA-MCF disease in a morbid 50-days-old cattle calf (Bos taurus indicus) and asymptomatic infection in the in-contact reservoir hosts, sheep (Ovis aries), and goat (Capra hircus) housed on a farm located in the Southern India is reported. An OIE recommended SA-MCF type-specific PCR confirmed the etiological agent as OvHV-2. The genetic characterization and phylogenetic analyses based on the glycoprotein B (gB) gene indicate that three genetic variants of OvHV-2 had infected the animal cluster of this study. As the OvHV-2 infection eventually lead to the death of the cattle calf, and the fact that its gB sequence carried four unique amino acid substitutions (N169S, L594P, I645V, and V730A), an investigation of these substitutions impact on its stability and molecular flexibility was carried out. The mapping of these amino acid substitutions on the three-dimensional structure of gB coupled with supplementary investigations showed that these substitutions conveyed the molecular flexibility to the gB, at the cost of its stability. Future studies would be to investigate whether these gB substitutions have any impact on membrane fusion activity using a virus-free cell-to-cell membrane fusion assay. The study also highlights the importance of adopting stringent biosecurity measures where mixed animal farming is a common practice.
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spelling pubmed-78843112021-02-17 First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India Kumar, Naveen Sood, Richa Pateriya, Atul K. Venkatesakumar, E. Ramprabhu, R. Dixit, Roma Bhatia, Sandeep Singh, Vijendra Pal Front Vet Sci Veterinary Science Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever (SA-MCF), a highly fatal disease syndrome that predominantly affects susceptible hosts of the order Artiodactyla. In this study, an in-depth clinico-molecular investigation of SA-MCF disease in a morbid 50-days-old cattle calf (Bos taurus indicus) and asymptomatic infection in the in-contact reservoir hosts, sheep (Ovis aries), and goat (Capra hircus) housed on a farm located in the Southern India is reported. An OIE recommended SA-MCF type-specific PCR confirmed the etiological agent as OvHV-2. The genetic characterization and phylogenetic analyses based on the glycoprotein B (gB) gene indicate that three genetic variants of OvHV-2 had infected the animal cluster of this study. As the OvHV-2 infection eventually lead to the death of the cattle calf, and the fact that its gB sequence carried four unique amino acid substitutions (N169S, L594P, I645V, and V730A), an investigation of these substitutions impact on its stability and molecular flexibility was carried out. The mapping of these amino acid substitutions on the three-dimensional structure of gB coupled with supplementary investigations showed that these substitutions conveyed the molecular flexibility to the gB, at the cost of its stability. Future studies would be to investigate whether these gB substitutions have any impact on membrane fusion activity using a virus-free cell-to-cell membrane fusion assay. The study also highlights the importance of adopting stringent biosecurity measures where mixed animal farming is a common practice. Frontiers Media S.A. 2021-02-02 /pmc/articles/PMC7884311/ /pubmed/33604365 http://dx.doi.org/10.3389/fvets.2021.610178 Text en Copyright © 2021 Kumar, Sood, Pateriya, Venkatesakumar, Ramprabhu, Dixit, Bhatia and Singh. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Kumar, Naveen
Sood, Richa
Pateriya, Atul K.
Venkatesakumar, E.
Ramprabhu, R.
Dixit, Roma
Bhatia, Sandeep
Singh, Vijendra Pal
First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India
title First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India
title_full First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India
title_fullStr First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India
title_full_unstemmed First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India
title_short First Molecular Evidence and Genetic Characterization of Ovine Herpesvirus 2 in Multiple Animal Species in India
title_sort first molecular evidence and genetic characterization of ovine herpesvirus 2 in multiple animal species in india
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884311/
https://www.ncbi.nlm.nih.gov/pubmed/33604365
http://dx.doi.org/10.3389/fvets.2021.610178
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