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Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
Hematological analysis is essential for patients who are supported by a mechanical circulatory support (MCS). The laboratory methods used to analyze blood components are conventional and accurate, but they require a mandatory turn-around-time for laboratory results, and because of toxic substances,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884396/ https://www.ncbi.nlm.nih.gov/pubmed/33589647 http://dx.doi.org/10.1038/s41598-021-83327-5 |
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author | Shin, Dong Ah Lee, Jung Chan Shin, Heean Cho, Young-Jae Kim, Hee Chan |
author_facet | Shin, Dong Ah Lee, Jung Chan Shin, Heean Cho, Young-Jae Kim, Hee Chan |
author_sort | Shin, Dong Ah |
collection | PubMed |
description | Hematological analysis is essential for patients who are supported by a mechanical circulatory support (MCS). The laboratory methods used to analyze blood components are conventional and accurate, but they require a mandatory turn-around-time for laboratory results, and because of toxic substances, can also be hazardous to analysis workers. Here, a simple and rapid point-of-care device is developed for the measurement of plasma free hemoglobin (PFHb) and hematocrit (Hct), based on colorimetry. The device consists of camera module, minimized centrifuge system, and the custom software that includes the motor control algorithm for the centrifuge system, and the image processing algorithm for measuring the color components of blood from the images. We show that our device measured PFHb with a detection limit of 0.75 mg/dL in the range of (0–100) mg/dL, and Hct with a detection limit of 2.14% in the range of (20–50)%. Our device had a high correlation with the measurement method generally used in clinical laboratories (PFHb R = 0.999, Hct R = 0.739), and the quantitative analysis resulted in precision of 1.44 mg/dL for PFHb value of 14.5 mg/dL, 1.36 mg/dL for PFHb value of 53 mg/dL, and 1.24% for Hct 30%. Also, the device can be measured without any pre-processing when compared to the clinical laboratory method, so results can be obtained within 5 min (about an 1 h for the clinical laboratory method). Therefore, we conclude that the device can be used for point-of-care measurement of PFHb and Hct for MCS. |
format | Online Article Text |
id | pubmed-7884396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78843962021-02-16 Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support Shin, Dong Ah Lee, Jung Chan Shin, Heean Cho, Young-Jae Kim, Hee Chan Sci Rep Article Hematological analysis is essential for patients who are supported by a mechanical circulatory support (MCS). The laboratory methods used to analyze blood components are conventional and accurate, but they require a mandatory turn-around-time for laboratory results, and because of toxic substances, can also be hazardous to analysis workers. Here, a simple and rapid point-of-care device is developed for the measurement of plasma free hemoglobin (PFHb) and hematocrit (Hct), based on colorimetry. The device consists of camera module, minimized centrifuge system, and the custom software that includes the motor control algorithm for the centrifuge system, and the image processing algorithm for measuring the color components of blood from the images. We show that our device measured PFHb with a detection limit of 0.75 mg/dL in the range of (0–100) mg/dL, and Hct with a detection limit of 2.14% in the range of (20–50)%. Our device had a high correlation with the measurement method generally used in clinical laboratories (PFHb R = 0.999, Hct R = 0.739), and the quantitative analysis resulted in precision of 1.44 mg/dL for PFHb value of 14.5 mg/dL, 1.36 mg/dL for PFHb value of 53 mg/dL, and 1.24% for Hct 30%. Also, the device can be measured without any pre-processing when compared to the clinical laboratory method, so results can be obtained within 5 min (about an 1 h for the clinical laboratory method). Therefore, we conclude that the device can be used for point-of-care measurement of PFHb and Hct for MCS. Nature Publishing Group UK 2021-02-15 /pmc/articles/PMC7884396/ /pubmed/33589647 http://dx.doi.org/10.1038/s41598-021-83327-5 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shin, Dong Ah Lee, Jung Chan Shin, Heean Cho, Young-Jae Kim, Hee Chan Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support |
title | Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support |
title_full | Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support |
title_fullStr | Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support |
title_full_unstemmed | Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support |
title_short | Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support |
title_sort | point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884396/ https://www.ncbi.nlm.nih.gov/pubmed/33589647 http://dx.doi.org/10.1038/s41598-021-83327-5 |
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