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PGMD/curcumin nanoparticles for the treatment of breast cancer

The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CU...

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Autores principales: Kumari, Mankamna, Sharma, Nikita, Manchanda, Romila, Gupta, Nidhi, Syed, Asad, Bahkali, Ali H., Nimesh, Surendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884397/
https://www.ncbi.nlm.nih.gov/pubmed/33589661
http://dx.doi.org/10.1038/s41598-021-81701-x
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author Kumari, Mankamna
Sharma, Nikita
Manchanda, Romila
Gupta, Nidhi
Syed, Asad
Bahkali, Ali H.
Nimesh, Surendra
author_facet Kumari, Mankamna
Sharma, Nikita
Manchanda, Romila
Gupta, Nidhi
Syed, Asad
Bahkali, Ali H.
Nimesh, Surendra
author_sort Kumari, Mankamna
collection PubMed
description The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was − 18.9 and − 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75–81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC(50) of the nanoformulations was observed to be 40.2 and 33.6 μM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 μM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis.
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spelling pubmed-78843972021-02-16 PGMD/curcumin nanoparticles for the treatment of breast cancer Kumari, Mankamna Sharma, Nikita Manchanda, Romila Gupta, Nidhi Syed, Asad Bahkali, Ali H. Nimesh, Surendra Sci Rep Article The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was − 18.9 and − 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75–81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC(50) of the nanoformulations was observed to be 40.2 and 33.6 μM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 μM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis. Nature Publishing Group UK 2021-02-15 /pmc/articles/PMC7884397/ /pubmed/33589661 http://dx.doi.org/10.1038/s41598-021-81701-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kumari, Mankamna
Sharma, Nikita
Manchanda, Romila
Gupta, Nidhi
Syed, Asad
Bahkali, Ali H.
Nimesh, Surendra
PGMD/curcumin nanoparticles for the treatment of breast cancer
title PGMD/curcumin nanoparticles for the treatment of breast cancer
title_full PGMD/curcumin nanoparticles for the treatment of breast cancer
title_fullStr PGMD/curcumin nanoparticles for the treatment of breast cancer
title_full_unstemmed PGMD/curcumin nanoparticles for the treatment of breast cancer
title_short PGMD/curcumin nanoparticles for the treatment of breast cancer
title_sort pgmd/curcumin nanoparticles for the treatment of breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884397/
https://www.ncbi.nlm.nih.gov/pubmed/33589661
http://dx.doi.org/10.1038/s41598-021-81701-x
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