The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs

Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgravity and...

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Autores principales: Dong, Jingjing, Wang, Honghui, Li, Gaozhi, Wang, Ke, Tan, Yingjun, Zhang, Lijun, Wang, Yixuan, Hu, Zebing, Cao, Xinsheng, Shi, Fei, Zhang, Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884416/
https://www.ncbi.nlm.nih.gov/pubmed/33589605
http://dx.doi.org/10.1038/s41526-021-00131-1
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author Dong, Jingjing
Wang, Honghui
Li, Gaozhi
Wang, Ke
Tan, Yingjun
Zhang, Lijun
Wang, Yixuan
Hu, Zebing
Cao, Xinsheng
Shi, Fei
Zhang, Shu
author_facet Dong, Jingjing
Wang, Honghui
Li, Gaozhi
Wang, Ke
Tan, Yingjun
Zhang, Lijun
Wang, Yixuan
Hu, Zebing
Cao, Xinsheng
Shi, Fei
Zhang, Shu
author_sort Dong, Jingjing
collection PubMed
description Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgravity and X-ray radiation, alone or combination, show decreased cell activity, increased apoptosis rates by flow cytometric analysis, and decreased Runx2 and increased Caspase-3 mRNA and protein expressions. In rat femurs, simulated microgravity and X-ray radiation, alone or combination, show increased bone loss by micro-CT test and Masson staining, decreased serum BALP levels and Runx2 mRNA expressions, and increased serum CTX-1 levels and Caspase-3 mRNA expressions. The strongest effect is observed in the combined group in MC3T3-E1 cells and rat femurs. These findings suggest that the combination of microgravity and radiation exacerbates the effects of either treatment alone on MC3T3-E1 cells and rat femurs.
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spelling pubmed-78844162021-02-25 The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs Dong, Jingjing Wang, Honghui Li, Gaozhi Wang, Ke Tan, Yingjun Zhang, Lijun Wang, Yixuan Hu, Zebing Cao, Xinsheng Shi, Fei Zhang, Shu NPJ Microgravity Article Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgravity and X-ray radiation, alone or combination, show decreased cell activity, increased apoptosis rates by flow cytometric analysis, and decreased Runx2 and increased Caspase-3 mRNA and protein expressions. In rat femurs, simulated microgravity and X-ray radiation, alone or combination, show increased bone loss by micro-CT test and Masson staining, decreased serum BALP levels and Runx2 mRNA expressions, and increased serum CTX-1 levels and Caspase-3 mRNA expressions. The strongest effect is observed in the combined group in MC3T3-E1 cells and rat femurs. These findings suggest that the combination of microgravity and radiation exacerbates the effects of either treatment alone on MC3T3-E1 cells and rat femurs. Nature Publishing Group UK 2021-02-15 /pmc/articles/PMC7884416/ /pubmed/33589605 http://dx.doi.org/10.1038/s41526-021-00131-1 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dong, Jingjing
Wang, Honghui
Li, Gaozhi
Wang, Ke
Tan, Yingjun
Zhang, Lijun
Wang, Yixuan
Hu, Zebing
Cao, Xinsheng
Shi, Fei
Zhang, Shu
The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs
title The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs
title_full The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs
title_fullStr The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs
title_full_unstemmed The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs
title_short The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs
title_sort combined effects of simulated microgravity and x-ray radiation on mc3t3-e1 cells and rat femurs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884416/
https://www.ncbi.nlm.nih.gov/pubmed/33589605
http://dx.doi.org/10.1038/s41526-021-00131-1
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