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Proteome-wide and matrisome-specific alterations during human pancreas development and maturation

The extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quan...

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Autores principales: Li, Zihui, Tremmel, Daniel M., Ma, Fengfei, Yu, Qinying, Ma, Min, Delafield, Daniel G., Shi, Yatao, Wang, Bin, Mitchell, Samantha A., Feeney, Austin K., Jain, Vansh S., Sackett, Sara Dutton, Odorico, Jon S., Li, Lingjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884717/
https://www.ncbi.nlm.nih.gov/pubmed/33589611
http://dx.doi.org/10.1038/s41467-021-21261-w
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author Li, Zihui
Tremmel, Daniel M.
Ma, Fengfei
Yu, Qinying
Ma, Min
Delafield, Daniel G.
Shi, Yatao
Wang, Bin
Mitchell, Samantha A.
Feeney, Austin K.
Jain, Vansh S.
Sackett, Sara Dutton
Odorico, Jon S.
Li, Lingjun
author_facet Li, Zihui
Tremmel, Daniel M.
Ma, Fengfei
Yu, Qinying
Ma, Min
Delafield, Daniel G.
Shi, Yatao
Wang, Bin
Mitchell, Samantha A.
Feeney, Austin K.
Jain, Vansh S.
Sackett, Sara Dutton
Odorico, Jon S.
Li, Lingjun
author_sort Li, Zihui
collection PubMed
description The extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quantitative proteomics strategies using N,N-dimethyl leucine isobaric tags to delineate proteome-wide and ECM-specific alterations in four age groups: fetal (18-20 weeks gestation), juvenile (5-16 years old), young adults (21-29 years old) and older adults (50-61 years old). We identify 3,523 proteins including 185 ECM proteins and quantify 117 of them. We detect previously unknown proteome and matrisome features during pancreas development and maturation. We also visualize specific ECM proteins of interest using immunofluorescent staining and investigate changes in ECM localization within islet or acinar compartments. This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation.
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spelling pubmed-78847172021-02-25 Proteome-wide and matrisome-specific alterations during human pancreas development and maturation Li, Zihui Tremmel, Daniel M. Ma, Fengfei Yu, Qinying Ma, Min Delafield, Daniel G. Shi, Yatao Wang, Bin Mitchell, Samantha A. Feeney, Austin K. Jain, Vansh S. Sackett, Sara Dutton Odorico, Jon S. Li, Lingjun Nat Commun Article The extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quantitative proteomics strategies using N,N-dimethyl leucine isobaric tags to delineate proteome-wide and ECM-specific alterations in four age groups: fetal (18-20 weeks gestation), juvenile (5-16 years old), young adults (21-29 years old) and older adults (50-61 years old). We identify 3,523 proteins including 185 ECM proteins and quantify 117 of them. We detect previously unknown proteome and matrisome features during pancreas development and maturation. We also visualize specific ECM proteins of interest using immunofluorescent staining and investigate changes in ECM localization within islet or acinar compartments. This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation. Nature Publishing Group UK 2021-02-15 /pmc/articles/PMC7884717/ /pubmed/33589611 http://dx.doi.org/10.1038/s41467-021-21261-w Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Zihui
Tremmel, Daniel M.
Ma, Fengfei
Yu, Qinying
Ma, Min
Delafield, Daniel G.
Shi, Yatao
Wang, Bin
Mitchell, Samantha A.
Feeney, Austin K.
Jain, Vansh S.
Sackett, Sara Dutton
Odorico, Jon S.
Li, Lingjun
Proteome-wide and matrisome-specific alterations during human pancreas development and maturation
title Proteome-wide and matrisome-specific alterations during human pancreas development and maturation
title_full Proteome-wide and matrisome-specific alterations during human pancreas development and maturation
title_fullStr Proteome-wide and matrisome-specific alterations during human pancreas development and maturation
title_full_unstemmed Proteome-wide and matrisome-specific alterations during human pancreas development and maturation
title_short Proteome-wide and matrisome-specific alterations during human pancreas development and maturation
title_sort proteome-wide and matrisome-specific alterations during human pancreas development and maturation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884717/
https://www.ncbi.nlm.nih.gov/pubmed/33589611
http://dx.doi.org/10.1038/s41467-021-21261-w
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