Cargando…
An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0
Portulaca oleracea L., known as the “vegetable for long life,” is an annual succulent herb that is widely distributed worldwide. Many clinical and experimental studies have demonstrated that purslane seed (MCXZ) can be used as an adjunctive and alternative therapy for the treatment of diabetes melli...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884824/ https://www.ncbi.nlm.nih.gov/pubmed/33603663 http://dx.doi.org/10.3389/fphar.2020.593693 |
_version_ | 1783651493174312960 |
---|---|
author | Hou, Jinli Zhou, Xiang Wang, Ping Zhao, Chunhui Qin, Yuewen Liu, Feng Yu, Liping Xu, Haiyu |
author_facet | Hou, Jinli Zhou, Xiang Wang, Ping Zhao, Chunhui Qin, Yuewen Liu, Feng Yu, Liping Xu, Haiyu |
author_sort | Hou, Jinli |
collection | PubMed |
description | Portulaca oleracea L., known as the “vegetable for long life,” is an annual succulent herb that is widely distributed worldwide. Many clinical and experimental studies have demonstrated that purslane seed (MCXZ) can be used as an adjunctive and alternative therapy for the treatment of diabetes mellitus (DM). However, the underlying active constituents and pharmacological mechanisms through which MCXZ exerts effects in DM remain unclear. In the present study, we confirmed that MCXZ treatment resulted in hypoglycemic activity, lowering the fasting blood glucose and glycated hemoglobin levels in streptozotocin-induced diabetic mice. Then, ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry was used to systematically analyze the chemical profile of MCXZ, resulting in the identification of 84 constituents, including 31 organic acids and nine flavonoids. Finally, the Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine was employed to analyze the key active components of MCXZ and the molecular mechanisms through which these components acted in DM. Ten key active compounds were identified based on the topological importance of their corresponding putative targets within the known DM-associated therapeutic target network of known MCXZ putative targets. Functionally, these candidate targets play critical anti-hyperlipidemia, anti-hyperglycemia, immunity regulation, and inflammatory roles involving DM-related pathways, such as the vascular endothelial growth factor (VEGF) signaling pathway and Fc gamma R-mediated phagocytosis, which indicated that MCXZ exhibited anti-diabetic activity through multi-faced actions. |
format | Online Article Text |
id | pubmed-7884824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78848242021-02-17 An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0 Hou, Jinli Zhou, Xiang Wang, Ping Zhao, Chunhui Qin, Yuewen Liu, Feng Yu, Liping Xu, Haiyu Front Pharmacol Pharmacology Portulaca oleracea L., known as the “vegetable for long life,” is an annual succulent herb that is widely distributed worldwide. Many clinical and experimental studies have demonstrated that purslane seed (MCXZ) can be used as an adjunctive and alternative therapy for the treatment of diabetes mellitus (DM). However, the underlying active constituents and pharmacological mechanisms through which MCXZ exerts effects in DM remain unclear. In the present study, we confirmed that MCXZ treatment resulted in hypoglycemic activity, lowering the fasting blood glucose and glycated hemoglobin levels in streptozotocin-induced diabetic mice. Then, ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry was used to systematically analyze the chemical profile of MCXZ, resulting in the identification of 84 constituents, including 31 organic acids and nine flavonoids. Finally, the Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine was employed to analyze the key active components of MCXZ and the molecular mechanisms through which these components acted in DM. Ten key active compounds were identified based on the topological importance of their corresponding putative targets within the known DM-associated therapeutic target network of known MCXZ putative targets. Functionally, these candidate targets play critical anti-hyperlipidemia, anti-hyperglycemia, immunity regulation, and inflammatory roles involving DM-related pathways, such as the vascular endothelial growth factor (VEGF) signaling pathway and Fc gamma R-mediated phagocytosis, which indicated that MCXZ exhibited anti-diabetic activity through multi-faced actions. Frontiers Media S.A. 2021-02-02 /pmc/articles/PMC7884824/ /pubmed/33603663 http://dx.doi.org/10.3389/fphar.2020.593693 Text en Copyright © 2021 Hou, Zhou, Wang, Zhao, Qin, Liu, Yu and Xu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Hou, Jinli Zhou, Xiang Wang, Ping Zhao, Chunhui Qin, Yuewen Liu, Feng Yu, Liping Xu, Haiyu An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0 |
title | An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0 |
title_full | An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0 |
title_fullStr | An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0 |
title_full_unstemmed | An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0 |
title_short | An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0 |
title_sort | integrative pharmacology-based approach for evaluating the potential effects of purslane seed in diabetes mellitus treatment using uhplc-ltq-orbitrap and tcmip v2.0 |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884824/ https://www.ncbi.nlm.nih.gov/pubmed/33603663 http://dx.doi.org/10.3389/fphar.2020.593693 |
work_keys_str_mv | AT houjinli anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT zhouxiang anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT wangping anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT zhaochunhui anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT qinyuewen anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT liufeng anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT yuliping anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT xuhaiyu anintegrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT houjinli integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT zhouxiang integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT wangping integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT zhaochunhui integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT qinyuewen integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT liufeng integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT yuliping integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 AT xuhaiyu integrativepharmacologybasedapproachforevaluatingthepotentialeffectsofpurslaneseedindiabetesmellitustreatmentusinguhplcltqorbitrapandtcmipv20 |