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Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)

Snake venom contains a variety of toxins with a range of biological activity, among these toxins cysteine-rich secreted proteins (CRISPs) can be found. The proteins of this family have masses of 20–30 kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disu...

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Autores principales: Badari, Juliana Cuoco, Díaz-Roa, Andrea, Teixeira Rocha, Marisa Maria, Mendonça, Ronaldo Zucatelli, da Silva Junior, Pedro Ismael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884886/
https://www.ncbi.nlm.nih.gov/pubmed/33603660
http://dx.doi.org/10.3389/fphar.2020.586705
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author Badari, Juliana Cuoco
Díaz-Roa, Andrea
Teixeira Rocha, Marisa Maria
Mendonça, Ronaldo Zucatelli
da Silva Junior, Pedro Ismael
author_facet Badari, Juliana Cuoco
Díaz-Roa, Andrea
Teixeira Rocha, Marisa Maria
Mendonça, Ronaldo Zucatelli
da Silva Junior, Pedro Ismael
author_sort Badari, Juliana Cuoco
collection PubMed
description Snake venom contains a variety of toxins with a range of biological activity, among these toxins cysteine-rich secreted proteins (CRISPs) can be found. The proteins of this family have masses of 20–30 kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disulfide bonds. Some of these proteins have been explored, characterized, and described in terms of their activity; however, little is known about their range of activities. A search for new antimicrobial molecules is ongoing, as the number of microbial strains resistant to available antibiotics is increasing. We identified antimicrobial activity in the secretion of Duvernoy's gland of the rear-fanged Philodryas patagoniensis. Fractions of this venom were subjected to reverse-phase high performance liquid chromatography and analyzed to determine their antimicrobial activity with a liquid broth inhibition assay. One of the fractions presented activity against a Gram-negative bacterium and a filamentous fungus. This fraction was analyzed with LC-MS/MS, and a protein of 24,848.8 Da was identified. Database searches allowed us to identify it as a CRISP due to the presence of some unique fragments in the molecule. We called it patagonin-CRISP, as the same protein in the venom of P. patagoniensis had previously been characterized as having a different biological activity. Patagonin-CRISP presented activity at very low concentrations and showed no cytotoxic activity. This is the first time that antimicrobial activity has been identified for P. patagoniensis venom or for a CRISP family protein.
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spelling pubmed-78848862021-02-17 Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake) Badari, Juliana Cuoco Díaz-Roa, Andrea Teixeira Rocha, Marisa Maria Mendonça, Ronaldo Zucatelli da Silva Junior, Pedro Ismael Front Pharmacol Pharmacology Snake venom contains a variety of toxins with a range of biological activity, among these toxins cysteine-rich secreted proteins (CRISPs) can be found. The proteins of this family have masses of 20–30 kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disulfide bonds. Some of these proteins have been explored, characterized, and described in terms of their activity; however, little is known about their range of activities. A search for new antimicrobial molecules is ongoing, as the number of microbial strains resistant to available antibiotics is increasing. We identified antimicrobial activity in the secretion of Duvernoy's gland of the rear-fanged Philodryas patagoniensis. Fractions of this venom were subjected to reverse-phase high performance liquid chromatography and analyzed to determine their antimicrobial activity with a liquid broth inhibition assay. One of the fractions presented activity against a Gram-negative bacterium and a filamentous fungus. This fraction was analyzed with LC-MS/MS, and a protein of 24,848.8 Da was identified. Database searches allowed us to identify it as a CRISP due to the presence of some unique fragments in the molecule. We called it patagonin-CRISP, as the same protein in the venom of P. patagoniensis had previously been characterized as having a different biological activity. Patagonin-CRISP presented activity at very low concentrations and showed no cytotoxic activity. This is the first time that antimicrobial activity has been identified for P. patagoniensis venom or for a CRISP family protein. Frontiers Media S.A. 2021-02-02 /pmc/articles/PMC7884886/ /pubmed/33603660 http://dx.doi.org/10.3389/fphar.2020.586705 Text en Copyright © 2021 Badari, Daz-Roa, Teixeira Rocha, Mendonça and Silva Junior. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Badari, Juliana Cuoco
Díaz-Roa, Andrea
Teixeira Rocha, Marisa Maria
Mendonça, Ronaldo Zucatelli
da Silva Junior, Pedro Ismael
Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)
title Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)
title_full Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)
title_fullStr Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)
title_full_unstemmed Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)
title_short Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)
title_sort patagonin-crisp: antimicrobial activity and source of antimicrobial molecules in duvernoy’s gland secretion (philodryas patagoniensis snake)
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884886/
https://www.ncbi.nlm.nih.gov/pubmed/33603660
http://dx.doi.org/10.3389/fphar.2020.586705
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