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Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract

The human gastrointestinal mucosal surface consists of a eukaryotic epithelium, a prokaryotic microbiota, and a carbohydrate-rich interface that separates them. In the gastrointestinal tract, the interaction of bacteriophages (phages) and their prokaryotic hosts influences the health of the mammalia...

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Autores principales: Green, Sabrina I., Gu Liu, Carmen, Yu, Xue, Gibson, Shelley, Salmen, Wilhem, Rajan, Anubama, Carter, Hannah E., Clark, Justin R., Song, Xuezheng, Ramig, Robert F., Trautner, Barbara W., Kaplan, Heidi B., Maresso, Anthony W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885116/
https://www.ncbi.nlm.nih.gov/pubmed/33563833
http://dx.doi.org/10.1128/mBio.03474-20
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author Green, Sabrina I.
Gu Liu, Carmen
Yu, Xue
Gibson, Shelley
Salmen, Wilhem
Rajan, Anubama
Carter, Hannah E.
Clark, Justin R.
Song, Xuezheng
Ramig, Robert F.
Trautner, Barbara W.
Kaplan, Heidi B.
Maresso, Anthony W.
author_facet Green, Sabrina I.
Gu Liu, Carmen
Yu, Xue
Gibson, Shelley
Salmen, Wilhem
Rajan, Anubama
Carter, Hannah E.
Clark, Justin R.
Song, Xuezheng
Ramig, Robert F.
Trautner, Barbara W.
Kaplan, Heidi B.
Maresso, Anthony W.
author_sort Green, Sabrina I.
collection PubMed
description The human gastrointestinal mucosal surface consists of a eukaryotic epithelium, a prokaryotic microbiota, and a carbohydrate-rich interface that separates them. In the gastrointestinal tract, the interaction of bacteriophages (phages) and their prokaryotic hosts influences the health of the mammalian host, especially colonization with invasive pathobionts. Antibiotics may be used, but they also kill protective commensals. Here, we report a novel phage whose lytic cycle is enhanced in intestinal environments. The tail fiber gene, whose protein product binds human heparan sulfated proteoglycans and localizes the phage to the epithelial cell surface, positions it near its bacterial host, a type of locational targeting mechanism. This finding offers the prospect of developing mucosal targeting phage to selectively remove invasive pathobiont species from mucosal surfaces.
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spelling pubmed-78851162021-02-19 Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract Green, Sabrina I. Gu Liu, Carmen Yu, Xue Gibson, Shelley Salmen, Wilhem Rajan, Anubama Carter, Hannah E. Clark, Justin R. Song, Xuezheng Ramig, Robert F. Trautner, Barbara W. Kaplan, Heidi B. Maresso, Anthony W. mBio Research Article The human gastrointestinal mucosal surface consists of a eukaryotic epithelium, a prokaryotic microbiota, and a carbohydrate-rich interface that separates them. In the gastrointestinal tract, the interaction of bacteriophages (phages) and their prokaryotic hosts influences the health of the mammalian host, especially colonization with invasive pathobionts. Antibiotics may be used, but they also kill protective commensals. Here, we report a novel phage whose lytic cycle is enhanced in intestinal environments. The tail fiber gene, whose protein product binds human heparan sulfated proteoglycans and localizes the phage to the epithelial cell surface, positions it near its bacterial host, a type of locational targeting mechanism. This finding offers the prospect of developing mucosal targeting phage to selectively remove invasive pathobiont species from mucosal surfaces. American Society for Microbiology 2021-02-09 /pmc/articles/PMC7885116/ /pubmed/33563833 http://dx.doi.org/10.1128/mBio.03474-20 Text en Copyright © 2021 Green et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Green, Sabrina I.
Gu Liu, Carmen
Yu, Xue
Gibson, Shelley
Salmen, Wilhem
Rajan, Anubama
Carter, Hannah E.
Clark, Justin R.
Song, Xuezheng
Ramig, Robert F.
Trautner, Barbara W.
Kaplan, Heidi B.
Maresso, Anthony W.
Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract
title Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract
title_full Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract
title_fullStr Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract
title_full_unstemmed Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract
title_short Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract
title_sort targeting of mammalian glycans enhances phage predation in the gastrointestinal tract
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885116/
https://www.ncbi.nlm.nih.gov/pubmed/33563833
http://dx.doi.org/10.1128/mBio.03474-20
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