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Genetically Modified Rabbits for Cardiovascular Research

Rabbits are one of the most used experimental animals for investigating the mechanisms of human cardiovascular disease and lipid metabolism because they are phylogenetically closer to human than rodents (mice and rats). Cholesterol-fed wild-type rabbits were first used to study human atherosclerosis...

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Detalles Bibliográficos
Autores principales: Fan, Jianglin, Wang, Yanli, Chen, Y. Eugene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885269/
https://www.ncbi.nlm.nih.gov/pubmed/33603774
http://dx.doi.org/10.3389/fgene.2021.614379
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author Fan, Jianglin
Wang, Yanli
Chen, Y. Eugene
author_facet Fan, Jianglin
Wang, Yanli
Chen, Y. Eugene
author_sort Fan, Jianglin
collection PubMed
description Rabbits are one of the most used experimental animals for investigating the mechanisms of human cardiovascular disease and lipid metabolism because they are phylogenetically closer to human than rodents (mice and rats). Cholesterol-fed wild-type rabbits were first used to study human atherosclerosis more than 100 years ago and are still playing an important role in cardiovascular research. Furthermore, transgenic rabbits generated by pronuclear microinjection provided another means to investigate many gene functions associated with human disease. Because of the lack of both rabbit embryonic stem cells and the genome information, for a long time, it has been a dream for scientists to obtain knockout rabbits generated by homologous recombination-based genomic manipulation as in mice. This obstacle has greatly hampered using genetically modified rabbits to disclose the molecular mechanisms of many human diseases. The advent of genome editing technologies has dramatically extended the applications of experimental animals including rabbits. In this review, we will update genetically modified rabbits, including transgenic, knock-out, and knock-in rabbits during the past decades regarding their use in cardiovascular research and point out the perspectives in future.
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spelling pubmed-78852692021-02-17 Genetically Modified Rabbits for Cardiovascular Research Fan, Jianglin Wang, Yanli Chen, Y. Eugene Front Genet Genetics Rabbits are one of the most used experimental animals for investigating the mechanisms of human cardiovascular disease and lipid metabolism because they are phylogenetically closer to human than rodents (mice and rats). Cholesterol-fed wild-type rabbits were first used to study human atherosclerosis more than 100 years ago and are still playing an important role in cardiovascular research. Furthermore, transgenic rabbits generated by pronuclear microinjection provided another means to investigate many gene functions associated with human disease. Because of the lack of both rabbit embryonic stem cells and the genome information, for a long time, it has been a dream for scientists to obtain knockout rabbits generated by homologous recombination-based genomic manipulation as in mice. This obstacle has greatly hampered using genetically modified rabbits to disclose the molecular mechanisms of many human diseases. The advent of genome editing technologies has dramatically extended the applications of experimental animals including rabbits. In this review, we will update genetically modified rabbits, including transgenic, knock-out, and knock-in rabbits during the past decades regarding their use in cardiovascular research and point out the perspectives in future. Frontiers Media S.A. 2021-02-02 /pmc/articles/PMC7885269/ /pubmed/33603774 http://dx.doi.org/10.3389/fgene.2021.614379 Text en Copyright © 2021 Fan, Wang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Fan, Jianglin
Wang, Yanli
Chen, Y. Eugene
Genetically Modified Rabbits for Cardiovascular Research
title Genetically Modified Rabbits for Cardiovascular Research
title_full Genetically Modified Rabbits for Cardiovascular Research
title_fullStr Genetically Modified Rabbits for Cardiovascular Research
title_full_unstemmed Genetically Modified Rabbits for Cardiovascular Research
title_short Genetically Modified Rabbits for Cardiovascular Research
title_sort genetically modified rabbits for cardiovascular research
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885269/
https://www.ncbi.nlm.nih.gov/pubmed/33603774
http://dx.doi.org/10.3389/fgene.2021.614379
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