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Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018
Middle East Respiratory Syndrome coronavirus (MERS-CoV) is a coronavirus that infects both humans and dromedary camels and is responsible for an ongoing outbreak of severe respiratory illness in humans in the Middle East. While some mutations found in camel-derived MERS-CoV strains have been charact...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885919/ https://www.ncbi.nlm.nih.gov/pubmed/33594367 http://dx.doi.org/10.1101/2021.02.11.429193 |
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author | van Doremalen, Neeltje Letko, Michael Fischer, Robert J. Bushmaker, Trenton Yinda, Claude Kwe Schulz, Jonathan Seifert, Stephanie N. Kim, Nam Joong Hemida, Maged G Kayali, Ghazi Park, Wan Beom Perera, Ranawaka APM Tamin, Azaibi Thornburg, Natalie J. Tong, Suxiang Queen, Krista van Kerkhove, Maria D. Choi, Young Ki Oh, Myoung-don Assiri, Abdullah M. Peiris, Malik Gerber, Susan I. Munster, Vincent J. |
author_facet | van Doremalen, Neeltje Letko, Michael Fischer, Robert J. Bushmaker, Trenton Yinda, Claude Kwe Schulz, Jonathan Seifert, Stephanie N. Kim, Nam Joong Hemida, Maged G Kayali, Ghazi Park, Wan Beom Perera, Ranawaka APM Tamin, Azaibi Thornburg, Natalie J. Tong, Suxiang Queen, Krista van Kerkhove, Maria D. Choi, Young Ki Oh, Myoung-don Assiri, Abdullah M. Peiris, Malik Gerber, Susan I. Munster, Vincent J. |
author_sort | van Doremalen, Neeltje |
collection | PubMed |
description | Middle East Respiratory Syndrome coronavirus (MERS-CoV) is a coronavirus that infects both humans and dromedary camels and is responsible for an ongoing outbreak of severe respiratory illness in humans in the Middle East. While some mutations found in camel-derived MERS-CoV strains have been characterized, the majority of natural variation found across MERS-CoV isolates remains unstudied. Here we report on the environmental stability, replication kinetics and pathogenicity of several diverse isolates of MERS-CoV as well as SARS-CoV-2 to serve as a basis of comparison with other stability studies. While most of the MERS-CoV isolates exhibited similar stability and pathogenicity in our experiments, the camel derived isolate, C/KSA/13, exhibited reduced surface stability while another camel isolate, C/BF/15, had reduced pathogenicity in a small animal model. These results suggest that while betacoronaviruses may have similar environmental stability profiles, individual variation can influence this phenotype, underscoring the importance of continual, global viral surveillance. |
format | Online Article Text |
id | pubmed-7885919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-78859192021-02-17 Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018 van Doremalen, Neeltje Letko, Michael Fischer, Robert J. Bushmaker, Trenton Yinda, Claude Kwe Schulz, Jonathan Seifert, Stephanie N. Kim, Nam Joong Hemida, Maged G Kayali, Ghazi Park, Wan Beom Perera, Ranawaka APM Tamin, Azaibi Thornburg, Natalie J. Tong, Suxiang Queen, Krista van Kerkhove, Maria D. Choi, Young Ki Oh, Myoung-don Assiri, Abdullah M. Peiris, Malik Gerber, Susan I. Munster, Vincent J. bioRxiv Article Middle East Respiratory Syndrome coronavirus (MERS-CoV) is a coronavirus that infects both humans and dromedary camels and is responsible for an ongoing outbreak of severe respiratory illness in humans in the Middle East. While some mutations found in camel-derived MERS-CoV strains have been characterized, the majority of natural variation found across MERS-CoV isolates remains unstudied. Here we report on the environmental stability, replication kinetics and pathogenicity of several diverse isolates of MERS-CoV as well as SARS-CoV-2 to serve as a basis of comparison with other stability studies. While most of the MERS-CoV isolates exhibited similar stability and pathogenicity in our experiments, the camel derived isolate, C/KSA/13, exhibited reduced surface stability while another camel isolate, C/BF/15, had reduced pathogenicity in a small animal model. These results suggest that while betacoronaviruses may have similar environmental stability profiles, individual variation can influence this phenotype, underscoring the importance of continual, global viral surveillance. Cold Spring Harbor Laboratory 2021-02-12 /pmc/articles/PMC7885919/ /pubmed/33594367 http://dx.doi.org/10.1101/2021.02.11.429193 Text en https://creativecommons.org/publicdomain/zero/1.0/This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license (https://creativecommons.org/publicdomain/zero/1.0/) . |
spellingShingle | Article van Doremalen, Neeltje Letko, Michael Fischer, Robert J. Bushmaker, Trenton Yinda, Claude Kwe Schulz, Jonathan Seifert, Stephanie N. Kim, Nam Joong Hemida, Maged G Kayali, Ghazi Park, Wan Beom Perera, Ranawaka APM Tamin, Azaibi Thornburg, Natalie J. Tong, Suxiang Queen, Krista van Kerkhove, Maria D. Choi, Young Ki Oh, Myoung-don Assiri, Abdullah M. Peiris, Malik Gerber, Susan I. Munster, Vincent J. Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018 |
title | Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018 |
title_full | Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018 |
title_fullStr | Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018 |
title_full_unstemmed | Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018 |
title_short | Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018 |
title_sort | surface-aerosol stability and pathogenicity of diverse mers-cov strains from 2012 – 2018 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885919/ https://www.ncbi.nlm.nih.gov/pubmed/33594367 http://dx.doi.org/10.1101/2021.02.11.429193 |
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