Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019....

Descripción completa

Detalles Bibliográficos
Autores principales: Kaku, Norihito, Hashiguchi, Kohji, Akamatsu, Norihiko, Wakigawa, Fumiko, Matsuda, Junichi, Komaru, Kenzo, Nakao, Takumi, Harada, Yosuke, Hara, Atsuko, Uno, Naoki, Sakamoto, Kei, Morinaga, Yoshitomo, Kitazaki, Takeshi, Hasegawa, Hiroo, Miyazaki, Taiga, Fukuda, Masaaki, Izumikawa, Koichi, Mukae, Hiroshi, Yanagihara, Katsunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885976/
https://www.ncbi.nlm.nih.gov/pubmed/33594599
http://dx.doi.org/10.1007/s10096-021-04193-7
_version_ 1783651703601496064
author Kaku, Norihito
Hashiguchi, Kohji
Akamatsu, Norihiko
Wakigawa, Fumiko
Matsuda, Junichi
Komaru, Kenzo
Nakao, Takumi
Harada, Yosuke
Hara, Atsuko
Uno, Naoki
Sakamoto, Kei
Morinaga, Yoshitomo
Kitazaki, Takeshi
Hasegawa, Hiroo
Miyazaki, Taiga
Fukuda, Masaaki
Izumikawa, Koichi
Mukae, Hiroshi
Yanagihara, Katsunori
author_facet Kaku, Norihito
Hashiguchi, Kohji
Akamatsu, Norihiko
Wakigawa, Fumiko
Matsuda, Junichi
Komaru, Kenzo
Nakao, Takumi
Harada, Yosuke
Hara, Atsuko
Uno, Naoki
Sakamoto, Kei
Morinaga, Yoshitomo
Kitazaki, Takeshi
Hasegawa, Hiroo
Miyazaki, Taiga
Fukuda, Masaaki
Izumikawa, Koichi
Mukae, Hiroshi
Yanagihara, Katsunori
author_sort Kaku, Norihito
collection PubMed
description We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.
format Online
Article
Text
id pubmed-7885976
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-78859762021-02-17 Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples Kaku, Norihito Hashiguchi, Kohji Akamatsu, Norihiko Wakigawa, Fumiko Matsuda, Junichi Komaru, Kenzo Nakao, Takumi Harada, Yosuke Hara, Atsuko Uno, Naoki Sakamoto, Kei Morinaga, Yoshitomo Kitazaki, Takeshi Hasegawa, Hiroo Miyazaki, Taiga Fukuda, Masaaki Izumikawa, Koichi Mukae, Hiroshi Yanagihara, Katsunori Eur J Clin Microbiol Infect Dis Brief Report We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses. Springer Berlin Heidelberg 2021-02-16 2021 /pmc/articles/PMC7885976/ /pubmed/33594599 http://dx.doi.org/10.1007/s10096-021-04193-7 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Kaku, Norihito
Hashiguchi, Kohji
Akamatsu, Norihiko
Wakigawa, Fumiko
Matsuda, Junichi
Komaru, Kenzo
Nakao, Takumi
Harada, Yosuke
Hara, Atsuko
Uno, Naoki
Sakamoto, Kei
Morinaga, Yoshitomo
Kitazaki, Takeshi
Hasegawa, Hiroo
Miyazaki, Taiga
Fukuda, Masaaki
Izumikawa, Koichi
Mukae, Hiroshi
Yanagihara, Katsunori
Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
title Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
title_full Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
title_fullStr Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
title_full_unstemmed Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
title_short Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
title_sort evaluation of a novel rapid trc assay for the detection of influenza using nasopharyngeal swabs and gargle samples
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885976/
https://www.ncbi.nlm.nih.gov/pubmed/33594599
http://dx.doi.org/10.1007/s10096-021-04193-7
work_keys_str_mv AT kakunorihito evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT hashiguchikohji evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT akamatsunorihiko evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT wakigawafumiko evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT matsudajunichi evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT komarukenzo evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT nakaotakumi evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT haradayosuke evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT haraatsuko evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT unonaoki evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT sakamotokei evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT morinagayoshitomo evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT kitazakitakeshi evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT hasegawahiroo evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT miyazakitaiga evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT fukudamasaaki evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT izumikawakoichi evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT mukaehiroshi evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples
AT yanagiharakatsunori evaluationofanovelrapidtrcassayforthedetectionofinfluenzausingnasopharyngealswabsandgarglesamples

Ejemplares similares