Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV...

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Autores principales: Bong, Ji-Hong, Kim, Tae-Hun, Jung, Jaeyong, Lee, Soo Jeong, Sung, Jeong Soo, Lee, Chang Kyu, Kang, Min-Jung, Kim, Hyun Ok, Pyun, Jae-Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean BioChip Society (KBCS) 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887419/
https://www.ncbi.nlm.nih.gov/pubmed/33613853
http://dx.doi.org/10.1007/s13206-021-00011-6
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author Bong, Ji-Hong
Kim, Tae-Hun
Jung, Jaeyong
Lee, Soo Jeong
Sung, Jeong Soo
Lee, Chang Kyu
Kang, Min-Jung
Kim, Hyun Ok
Pyun, Jae-Chul
author_facet Bong, Ji-Hong
Kim, Tae-Hun
Jung, Jaeyong
Lee, Soo Jeong
Sung, Jeong Soo
Lee, Chang Kyu
Kang, Min-Jung
Kim, Hyun Ok
Pyun, Jae-Chul
author_sort Bong, Ji-Hong
collection PubMed
description Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.
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spelling pubmed-78874192021-02-17 Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies Bong, Ji-Hong Kim, Tae-Hun Jung, Jaeyong Lee, Soo Jeong Sung, Jeong Soo Lee, Chang Kyu Kang, Min-Jung Kim, Hyun Ok Pyun, Jae-Chul Biochip J Original Article Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid. The Korean BioChip Society (KBCS) 2021-02-17 2021 /pmc/articles/PMC7887419/ /pubmed/33613853 http://dx.doi.org/10.1007/s13206-021-00011-6 Text en © The Korean BioChip Society 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Bong, Ji-Hong
Kim, Tae-Hun
Jung, Jaeyong
Lee, Soo Jeong
Sung, Jeong Soo
Lee, Chang Kyu
Kang, Min-Jung
Kim, Hyun Ok
Pyun, Jae-Chul
Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
title Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
title_full Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
title_fullStr Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
title_full_unstemmed Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
title_short Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies
title_sort competitive immunoassay of sars-cov-2 using pig sera-derived anti-sars-cov-2 antibodies
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887419/
https://www.ncbi.nlm.nih.gov/pubmed/33613853
http://dx.doi.org/10.1007/s13206-021-00011-6
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