Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model

The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch(wt/lox)) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation...

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Detalles Bibliográficos
Autores principales: Wong, Ho Yi, Khosrotehrani, Kiarash, Roy, Edwige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887426/
https://www.ncbi.nlm.nih.gov/pubmed/33644769
http://dx.doi.org/10.1016/j.xpro.2021.100329
Descripción
Sumario:The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch(wt/lox)) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation. The high-resolution three-dimensional confocal images are an excellent tool to visualize and quantify the distribution of skin cancers at a given time point. For complete details on the use and execution of this protocol, please refer to Roy et al. (2020).

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