Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model

The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch(wt/lox)) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation...

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Detalles Bibliográficos
Autores principales: Wong, Ho Yi, Khosrotehrani, Kiarash, Roy, Edwige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887426/
https://www.ncbi.nlm.nih.gov/pubmed/33644769
http://dx.doi.org/10.1016/j.xpro.2021.100329
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author Wong, Ho Yi
Khosrotehrani, Kiarash
Roy, Edwige
author_facet Wong, Ho Yi
Khosrotehrani, Kiarash
Roy, Edwige
author_sort Wong, Ho Yi
collection PubMed
description The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch(wt/lox)) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation. The high-resolution three-dimensional confocal images are an excellent tool to visualize and quantify the distribution of skin cancers at a given time point. For complete details on the use and execution of this protocol, please refer to Roy et al. (2020).
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spelling pubmed-78874262021-02-26 Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model Wong, Ho Yi Khosrotehrani, Kiarash Roy, Edwige STAR Protoc Protocol The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch(wt/lox)) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation. The high-resolution three-dimensional confocal images are an excellent tool to visualize and quantify the distribution of skin cancers at a given time point. For complete details on the use and execution of this protocol, please refer to Roy et al. (2020). Elsevier 2021-02-05 /pmc/articles/PMC7887426/ /pubmed/33644769 http://dx.doi.org/10.1016/j.xpro.2021.100329 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wong, Ho Yi
Khosrotehrani, Kiarash
Roy, Edwige
Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model
title Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model
title_full Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model
title_fullStr Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model
title_full_unstemmed Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model
title_short Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model
title_sort whole-mount staining coupled to a uv-inducible basal cell carcinoma murine model
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887426/
https://www.ncbi.nlm.nih.gov/pubmed/33644769
http://dx.doi.org/10.1016/j.xpro.2021.100329
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AT royedwige wholemountstainingcoupledtoauvinduciblebasalcellcarcinomamurinemodel

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