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Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models
The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887602/ https://www.ncbi.nlm.nih.gov/pubmed/33643799 http://dx.doi.org/10.1002/advs.202003129 |
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author | Blanco‐Fernandez, Barbara Gaspar, Vítor M. Engel, Elisabeth Mano, João F. |
author_facet | Blanco‐Fernandez, Barbara Gaspar, Vítor M. Engel, Elisabeth Mano, João F. |
author_sort | Blanco‐Fernandez, Barbara |
collection | PubMed |
description | The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics. |
format | Online Article Text |
id | pubmed-7887602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78876022021-02-26 Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models Blanco‐Fernandez, Barbara Gaspar, Vítor M. Engel, Elisabeth Mano, João F. Adv Sci (Weinh) Reviews The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics. John Wiley and Sons Inc. 2021-01-04 /pmc/articles/PMC7887602/ /pubmed/33643799 http://dx.doi.org/10.1002/advs.202003129 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Reviews Blanco‐Fernandez, Barbara Gaspar, Vítor M. Engel, Elisabeth Mano, João F. Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models |
title | Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models |
title_full | Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models |
title_fullStr | Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models |
title_full_unstemmed | Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models |
title_short | Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models |
title_sort | proteinaceous hydrogels for bioengineering advanced 3d tumor models |
topic | Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887602/ https://www.ncbi.nlm.nih.gov/pubmed/33643799 http://dx.doi.org/10.1002/advs.202003129 |
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