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Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to Wistar rats
Aromatase, an estrogen synthase, exists in the gastric parietal cells of Wistar rats. The stomach synthesizes large amounts of estrogens and secretes them into the portal vein. We have been particularly studying gastric estrogen synthesis using Wistar rats. However, estrogen synthesis in the stomach...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887622/ https://www.ncbi.nlm.nih.gov/pubmed/32981898 http://dx.doi.org/10.1538/expanim.20-0089 |
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author | Kobayashi, Hiroto Shirasawa, Nobuyuki Naito, Akira |
author_facet | Kobayashi, Hiroto Shirasawa, Nobuyuki Naito, Akira |
author_sort | Kobayashi, Hiroto |
collection | PubMed |
description | Aromatase, an estrogen synthase, exists in the gastric parietal cells of Wistar rats. The stomach synthesizes large amounts of estrogens and secretes them into the portal vein. We have been particularly studying gastric estrogen synthesis using Wistar rats. However, estrogen synthesis in the stomach of Sprague-Dawley (SD) rats, which are used as frequently as those of the Wistar strain, has not been clarified. We examined steroid synthesis in the stomach of SD rats using immunohistochemistry, in situ hybridization, Western blotting, real-time PCR, and LC-MS/MS. Aromatase also exists in the stomach of SD rats. Its distribution was not found to be different from that of Wistar rats. Results show that H(+)/K(+)-ATPase β-subunit and aromatase colocalized in double immunofluorescence staining. Each steroid synthase downstream from progesterone was present in the gastric mucosa. These results suggest that steroid hormones are synthesized in the parietal cells in the same pathway as Wistar rats. Although mRNA expression of steroid synthases were higher in SD, no significant difference was found in the amount of protein and each steroid hormone level in the portal vein. Although differences between strains might exist in steroid hormone synthesis, results show that SD rats are as useful as Wistar rats for gastric estrogen synthesis experimentation. |
format | Online Article Text |
id | pubmed-7887622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78876222021-02-19 Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to Wistar rats Kobayashi, Hiroto Shirasawa, Nobuyuki Naito, Akira Exp Anim Original Aromatase, an estrogen synthase, exists in the gastric parietal cells of Wistar rats. The stomach synthesizes large amounts of estrogens and secretes them into the portal vein. We have been particularly studying gastric estrogen synthesis using Wistar rats. However, estrogen synthesis in the stomach of Sprague-Dawley (SD) rats, which are used as frequently as those of the Wistar strain, has not been clarified. We examined steroid synthesis in the stomach of SD rats using immunohistochemistry, in situ hybridization, Western blotting, real-time PCR, and LC-MS/MS. Aromatase also exists in the stomach of SD rats. Its distribution was not found to be different from that of Wistar rats. Results show that H(+)/K(+)-ATPase β-subunit and aromatase colocalized in double immunofluorescence staining. Each steroid synthase downstream from progesterone was present in the gastric mucosa. These results suggest that steroid hormones are synthesized in the parietal cells in the same pathway as Wistar rats. Although mRNA expression of steroid synthases were higher in SD, no significant difference was found in the amount of protein and each steroid hormone level in the portal vein. Although differences between strains might exist in steroid hormone synthesis, results show that SD rats are as useful as Wistar rats for gastric estrogen synthesis experimentation. Japanese Association for Laboratory Animal Science 2020-09-25 2021 /pmc/articles/PMC7887622/ /pubmed/32981898 http://dx.doi.org/10.1538/expanim.20-0089 Text en ©2021 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Original Kobayashi, Hiroto Shirasawa, Nobuyuki Naito, Akira Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to Wistar rats |
title | Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to
Wistar rats |
title_full | Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to
Wistar rats |
title_fullStr | Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to
Wistar rats |
title_full_unstemmed | Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to
Wistar rats |
title_short | Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to
Wistar rats |
title_sort | estrogen synthesis in the stomach of sprague-dawley rats: comparison to
wistar rats |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887622/ https://www.ncbi.nlm.nih.gov/pubmed/32981898 http://dx.doi.org/10.1538/expanim.20-0089 |
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