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Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors

BACKGROUND: Gastric adenocarcinoma is associated with H. pylori infection and inflammation that can result in the dysbiosis of gastric microbiota. The association of intestinal microbiota with gastric adenocarcinoma subtypes or with gastric gastrointestinal stromal tumors (GIST) is however not well...

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Autores principales: Sarhadi, Virinder, Mathew, Binu, Kokkola, Arto, Karla, Tiina, Tikkanen, Milja, Rautelin, Hilpi, Lahti, Leo, Puolakkainen, Pauli, Knuutila, Sakari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888145/
https://www.ncbi.nlm.nih.gov/pubmed/33596997
http://dx.doi.org/10.1186/s13099-021-00403-x
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author Sarhadi, Virinder
Mathew, Binu
Kokkola, Arto
Karla, Tiina
Tikkanen, Milja
Rautelin, Hilpi
Lahti, Leo
Puolakkainen, Pauli
Knuutila, Sakari
author_facet Sarhadi, Virinder
Mathew, Binu
Kokkola, Arto
Karla, Tiina
Tikkanen, Milja
Rautelin, Hilpi
Lahti, Leo
Puolakkainen, Pauli
Knuutila, Sakari
author_sort Sarhadi, Virinder
collection PubMed
description BACKGROUND: Gastric adenocarcinoma is associated with H. pylori infection and inflammation that can result in the dysbiosis of gastric microbiota. The association of intestinal microbiota with gastric adenocarcinoma subtypes or with gastric gastrointestinal stromal tumors (GIST) is however not well known. Therefore, we performed 16S rRNA gene sequencing on DNA isolated from stool samples of Finnish patients and controls to study differences in microbiota among different histological subtypes of gastric adenocarcinoma, gastric GIST and healthy controls. RESULTS: We found that gut microbiota alpha diversity was lowest in diffuse adenocarcinoma patients, followed by intestinal type and GIST patients, although the differences were not significant compared to controls. Beta-diversity analysis however showed significant differences in microbiota composition for all subtypes compared to controls. Significantly higher abundance of Enterobacteriaceae was observed in both adenocarcinoma subtypes, whereas lower abundance of Bifidobacteriaceae was seen only in diffuse adenocarcinoma and of Oscillibacter in intestinal adenocarcinoma. Both GIST and adenocarcinoma patients had higher abundance of Enterobacteriaceae and lower abundance of Lactobacillaceae and Oscillibacter while lower abundance of Lachnoclostridium, Bifidobacterium, Parabacteroides and Barnesiella was seen only in the adenocarcinoma patients. CONCLUSIONS: Our analysis shows association of higher Enterobacteriaceae abundance with all types of gastric tumors. Therefore it could be potentially useful as a marker of gastric malignancies. Lower gut microbiota diversity might be indicative of poorly differentiated, invasive, advanced or aggressive tumors and could possibly be a prognostic marker for gastric tumors.
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spelling pubmed-78881452021-02-22 Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors Sarhadi, Virinder Mathew, Binu Kokkola, Arto Karla, Tiina Tikkanen, Milja Rautelin, Hilpi Lahti, Leo Puolakkainen, Pauli Knuutila, Sakari Gut Pathog Research BACKGROUND: Gastric adenocarcinoma is associated with H. pylori infection and inflammation that can result in the dysbiosis of gastric microbiota. The association of intestinal microbiota with gastric adenocarcinoma subtypes or with gastric gastrointestinal stromal tumors (GIST) is however not well known. Therefore, we performed 16S rRNA gene sequencing on DNA isolated from stool samples of Finnish patients and controls to study differences in microbiota among different histological subtypes of gastric adenocarcinoma, gastric GIST and healthy controls. RESULTS: We found that gut microbiota alpha diversity was lowest in diffuse adenocarcinoma patients, followed by intestinal type and GIST patients, although the differences were not significant compared to controls. Beta-diversity analysis however showed significant differences in microbiota composition for all subtypes compared to controls. Significantly higher abundance of Enterobacteriaceae was observed in both adenocarcinoma subtypes, whereas lower abundance of Bifidobacteriaceae was seen only in diffuse adenocarcinoma and of Oscillibacter in intestinal adenocarcinoma. Both GIST and adenocarcinoma patients had higher abundance of Enterobacteriaceae and lower abundance of Lactobacillaceae and Oscillibacter while lower abundance of Lachnoclostridium, Bifidobacterium, Parabacteroides and Barnesiella was seen only in the adenocarcinoma patients. CONCLUSIONS: Our analysis shows association of higher Enterobacteriaceae abundance with all types of gastric tumors. Therefore it could be potentially useful as a marker of gastric malignancies. Lower gut microbiota diversity might be indicative of poorly differentiated, invasive, advanced or aggressive tumors and could possibly be a prognostic marker for gastric tumors. BioMed Central 2021-02-17 /pmc/articles/PMC7888145/ /pubmed/33596997 http://dx.doi.org/10.1186/s13099-021-00403-x Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Sarhadi, Virinder
Mathew, Binu
Kokkola, Arto
Karla, Tiina
Tikkanen, Milja
Rautelin, Hilpi
Lahti, Leo
Puolakkainen, Pauli
Knuutila, Sakari
Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
title Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
title_full Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
title_fullStr Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
title_full_unstemmed Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
title_short Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
title_sort gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888145/
https://www.ncbi.nlm.nih.gov/pubmed/33596997
http://dx.doi.org/10.1186/s13099-021-00403-x
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