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A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
BACKGROUND: Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work was to evaluate a multiplex-PCR assay for th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888160/ https://www.ncbi.nlm.nih.gov/pubmed/33593278 http://dx.doi.org/10.1186/s12879-021-05885-3 |
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author | Schaumburg, Frieder Froböse, Neele Köck, Robin |
author_facet | Schaumburg, Frieder Froböse, Neele Köck, Robin |
author_sort | Schaumburg, Frieder |
collection | PubMed |
description | BACKGROUND: Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work was to evaluate a multiplex-PCR assay for the diagnosis of traveller’s diarrhoea (TD) in a case-control study and to assess the concordance with the BioFire® FilmArray® Gastrointestinal Panel. METHODS: Stool samples from cases (n = 61) and controls (n = 30) were collected during travel and analysed by the GI-EB Screening assay (Seegene) in a case-control study. The concordance with the BioFire® FilmArray® Gastrointestinal Panel was expressed as the proportion of participants in which both tests agreed in the category “detected” and “not detected”. RESULTS: None of the test-target organisms (Campylobacter spp., Clostridioides difficile toxin A/B, Salmonella spp., Shigella spp./enteroinvasive Escherichia coli, E. coli O157, Shiga toxin-producing E. coli, Yersinia enterocolitica) was significantly associated with TD GI-EB Screening assay. The GI-EB Screening assay had an agreement with the BioFire® FilmArray® of 86.8–100%. CONCLUSION: The selection of test-target organisms included in the GI-EB Screening assay appears inappropriate for the diagnostic work-up of TD as none of the detected pathogens was associated with TD. The GI-EB Screening assay had a good concordance with BioFire® FilmArray®. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-021-05885-3. |
format | Online Article Text |
id | pubmed-7888160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78881602021-02-22 A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea Schaumburg, Frieder Froböse, Neele Köck, Robin BMC Infect Dis Research Article BACKGROUND: Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work was to evaluate a multiplex-PCR assay for the diagnosis of traveller’s diarrhoea (TD) in a case-control study and to assess the concordance with the BioFire® FilmArray® Gastrointestinal Panel. METHODS: Stool samples from cases (n = 61) and controls (n = 30) were collected during travel and analysed by the GI-EB Screening assay (Seegene) in a case-control study. The concordance with the BioFire® FilmArray® Gastrointestinal Panel was expressed as the proportion of participants in which both tests agreed in the category “detected” and “not detected”. RESULTS: None of the test-target organisms (Campylobacter spp., Clostridioides difficile toxin A/B, Salmonella spp., Shigella spp./enteroinvasive Escherichia coli, E. coli O157, Shiga toxin-producing E. coli, Yersinia enterocolitica) was significantly associated with TD GI-EB Screening assay. The GI-EB Screening assay had an agreement with the BioFire® FilmArray® of 86.8–100%. CONCLUSION: The selection of test-target organisms included in the GI-EB Screening assay appears inappropriate for the diagnostic work-up of TD as none of the detected pathogens was associated with TD. The GI-EB Screening assay had a good concordance with BioFire® FilmArray®. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-021-05885-3. BioMed Central 2021-02-16 /pmc/articles/PMC7888160/ /pubmed/33593278 http://dx.doi.org/10.1186/s12879-021-05885-3 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Schaumburg, Frieder Froböse, Neele Köck, Robin A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea |
title | A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea |
title_full | A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea |
title_fullStr | A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea |
title_full_unstemmed | A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea |
title_short | A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea |
title_sort | comparison of two multiplex-pcr assays for the diagnosis of traveller’s diarrhoea |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888160/ https://www.ncbi.nlm.nih.gov/pubmed/33593278 http://dx.doi.org/10.1186/s12879-021-05885-3 |
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