Cargando…

A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea

BACKGROUND: Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work was to evaluate a multiplex-PCR assay for th...

Descripción completa

Detalles Bibliográficos
Autores principales: Schaumburg, Frieder, Froböse, Neele, Köck, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888160/
https://www.ncbi.nlm.nih.gov/pubmed/33593278
http://dx.doi.org/10.1186/s12879-021-05885-3
_version_ 1783652114299355136
author Schaumburg, Frieder
Froböse, Neele
Köck, Robin
author_facet Schaumburg, Frieder
Froböse, Neele
Köck, Robin
author_sort Schaumburg, Frieder
collection PubMed
description BACKGROUND: Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work was to evaluate a multiplex-PCR assay for the diagnosis of traveller’s diarrhoea (TD) in a case-control study and to assess the concordance with the BioFire® FilmArray® Gastrointestinal Panel. METHODS: Stool samples from cases (n = 61) and controls (n = 30) were collected during travel and analysed by the GI-EB Screening assay (Seegene) in a case-control study. The concordance with the BioFire® FilmArray® Gastrointestinal Panel was expressed as the proportion of participants in which both tests agreed in the category “detected” and “not detected”. RESULTS: None of the test-target organisms (Campylobacter spp., Clostridioides difficile toxin A/B, Salmonella spp., Shigella spp./enteroinvasive Escherichia coli, E. coli O157, Shiga toxin-producing E. coli, Yersinia enterocolitica) was significantly associated with TD GI-EB Screening assay. The GI-EB Screening assay had an agreement with the BioFire® FilmArray® of 86.8–100%. CONCLUSION: The selection of test-target organisms included in the GI-EB Screening assay appears inappropriate for the diagnostic work-up of TD as none of the detected pathogens was associated with TD. The GI-EB Screening assay had a good concordance with BioFire® FilmArray®. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-021-05885-3.
format Online
Article
Text
id pubmed-7888160
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-78881602021-02-22 A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea Schaumburg, Frieder Froböse, Neele Köck, Robin BMC Infect Dis Research Article BACKGROUND: Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work was to evaluate a multiplex-PCR assay for the diagnosis of traveller’s diarrhoea (TD) in a case-control study and to assess the concordance with the BioFire® FilmArray® Gastrointestinal Panel. METHODS: Stool samples from cases (n = 61) and controls (n = 30) were collected during travel and analysed by the GI-EB Screening assay (Seegene) in a case-control study. The concordance with the BioFire® FilmArray® Gastrointestinal Panel was expressed as the proportion of participants in which both tests agreed in the category “detected” and “not detected”. RESULTS: None of the test-target organisms (Campylobacter spp., Clostridioides difficile toxin A/B, Salmonella spp., Shigella spp./enteroinvasive Escherichia coli, E. coli O157, Shiga toxin-producing E. coli, Yersinia enterocolitica) was significantly associated with TD GI-EB Screening assay. The GI-EB Screening assay had an agreement with the BioFire® FilmArray® of 86.8–100%. CONCLUSION: The selection of test-target organisms included in the GI-EB Screening assay appears inappropriate for the diagnostic work-up of TD as none of the detected pathogens was associated with TD. The GI-EB Screening assay had a good concordance with BioFire® FilmArray®. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-021-05885-3. BioMed Central 2021-02-16 /pmc/articles/PMC7888160/ /pubmed/33593278 http://dx.doi.org/10.1186/s12879-021-05885-3 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Schaumburg, Frieder
Froböse, Neele
Köck, Robin
A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
title A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
title_full A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
title_fullStr A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
title_full_unstemmed A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
title_short A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
title_sort comparison of two multiplex-pcr assays for the diagnosis of traveller’s diarrhoea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888160/
https://www.ncbi.nlm.nih.gov/pubmed/33593278
http://dx.doi.org/10.1186/s12879-021-05885-3
work_keys_str_mv AT schaumburgfrieder acomparisonoftwomultiplexpcrassaysforthediagnosisoftravellersdiarrhoea
AT froboseneele acomparisonoftwomultiplexpcrassaysforthediagnosisoftravellersdiarrhoea
AT kockrobin acomparisonoftwomultiplexpcrassaysforthediagnosisoftravellersdiarrhoea
AT schaumburgfrieder comparisonoftwomultiplexpcrassaysforthediagnosisoftravellersdiarrhoea
AT froboseneele comparisonoftwomultiplexpcrassaysforthediagnosisoftravellersdiarrhoea
AT kockrobin comparisonoftwomultiplexpcrassaysforthediagnosisoftravellersdiarrhoea