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Genetic diversity of Ethiopian durum wheat landraces
Genetic diversity and population structure assessment in crops is essential for marker trait association, marker assisted breeding and crop germplasm conservation. We analyzed a set of 285 durum wheat accessions comprising 215 Ethiopian durum wheat landraces, 10 released durum wheat varieties, 10 ad...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888639/ https://www.ncbi.nlm.nih.gov/pubmed/33596260 http://dx.doi.org/10.1371/journal.pone.0247016 |
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author | Negisho, Kefyalew Shibru, Surafel Pillen, Klaus Ordon, Frank Wehner, Gwendolin |
author_facet | Negisho, Kefyalew Shibru, Surafel Pillen, Klaus Ordon, Frank Wehner, Gwendolin |
author_sort | Negisho, Kefyalew |
collection | PubMed |
description | Genetic diversity and population structure assessment in crops is essential for marker trait association, marker assisted breeding and crop germplasm conservation. We analyzed a set of 285 durum wheat accessions comprising 215 Ethiopian durum wheat landraces, 10 released durum wheat varieties, 10 advanced durum wheat lines from Ethiopia, and 50 durum wheat lines from CIMMYT. We investigated the genetic diversity and population structure for the complete panel as well as for the 215 landraces, separately based on 11,919 SNP markers with known physical positions. The whole panel was clustered into two populations representing on the one hand mainly the landraces, and on the other hand mainly released, advanced and CIMMYT lines. Further population structure analysis of the landraces uncovered 4 subgroups emphasizing the high degree of genetic diversity within Ethiopian durum landraces. Population structure based AMOVA for both sets unveiled significant (P < 0.001) variation between populations and within populations. Total variation within population accessions (81%, 76%) was higher than total variation between populations (19%, 24%) for both sets. Population structure analysis based genetic differentiation (FST) and gene flow (Nm) for the whole set and the Ethiopian landraces were 0.19 and 0.24, 1.04, and 0.81, respectively indicating high genetic differentiation and limited gene flow. Diversity indices verify that the landrace panel was more diverse with (I = 0.7, He = 0.46, uHe = 0.46) than the advanced lines (I = 0.6, He = 0.42, uHe = 0.42). Similarly, differences within the landrace clusters were observed. In summary a high genetic diversity within Ethiopian durum wheat landraces was detected, which may be a target for national and international wheat improvement programs to exploit valuable traits for biotic and abiotic stresses. |
format | Online Article Text |
id | pubmed-7888639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78886392021-02-25 Genetic diversity of Ethiopian durum wheat landraces Negisho, Kefyalew Shibru, Surafel Pillen, Klaus Ordon, Frank Wehner, Gwendolin PLoS One Research Article Genetic diversity and population structure assessment in crops is essential for marker trait association, marker assisted breeding and crop germplasm conservation. We analyzed a set of 285 durum wheat accessions comprising 215 Ethiopian durum wheat landraces, 10 released durum wheat varieties, 10 advanced durum wheat lines from Ethiopia, and 50 durum wheat lines from CIMMYT. We investigated the genetic diversity and population structure for the complete panel as well as for the 215 landraces, separately based on 11,919 SNP markers with known physical positions. The whole panel was clustered into two populations representing on the one hand mainly the landraces, and on the other hand mainly released, advanced and CIMMYT lines. Further population structure analysis of the landraces uncovered 4 subgroups emphasizing the high degree of genetic diversity within Ethiopian durum landraces. Population structure based AMOVA for both sets unveiled significant (P < 0.001) variation between populations and within populations. Total variation within population accessions (81%, 76%) was higher than total variation between populations (19%, 24%) for both sets. Population structure analysis based genetic differentiation (FST) and gene flow (Nm) for the whole set and the Ethiopian landraces were 0.19 and 0.24, 1.04, and 0.81, respectively indicating high genetic differentiation and limited gene flow. Diversity indices verify that the landrace panel was more diverse with (I = 0.7, He = 0.46, uHe = 0.46) than the advanced lines (I = 0.6, He = 0.42, uHe = 0.42). Similarly, differences within the landrace clusters were observed. In summary a high genetic diversity within Ethiopian durum wheat landraces was detected, which may be a target for national and international wheat improvement programs to exploit valuable traits for biotic and abiotic stresses. Public Library of Science 2021-02-17 /pmc/articles/PMC7888639/ /pubmed/33596260 http://dx.doi.org/10.1371/journal.pone.0247016 Text en © 2021 Negisho et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Negisho, Kefyalew Shibru, Surafel Pillen, Klaus Ordon, Frank Wehner, Gwendolin Genetic diversity of Ethiopian durum wheat landraces |
title | Genetic diversity of Ethiopian durum wheat landraces |
title_full | Genetic diversity of Ethiopian durum wheat landraces |
title_fullStr | Genetic diversity of Ethiopian durum wheat landraces |
title_full_unstemmed | Genetic diversity of Ethiopian durum wheat landraces |
title_short | Genetic diversity of Ethiopian durum wheat landraces |
title_sort | genetic diversity of ethiopian durum wheat landraces |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888639/ https://www.ncbi.nlm.nih.gov/pubmed/33596260 http://dx.doi.org/10.1371/journal.pone.0247016 |
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