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A pentameric protein ring with novel architecture is required for herpesviral packaging
Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly understood. Here, we present structures of two such accessory factors from the oncogenic herpesviruses...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889075/ https://www.ncbi.nlm.nih.gov/pubmed/33554858 http://dx.doi.org/10.7554/eLife.62261 |
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author | Didychuk, Allison L Gates, Stephanie N Gardner, Matthew R Strong, Lisa M Martin, Andreas Glaunsinger, Britt A |
author_facet | Didychuk, Allison L Gates, Stephanie N Gardner, Matthew R Strong, Lisa M Martin, Andreas Glaunsinger, Britt A |
author_sort | Didychuk, Allison L |
collection | PubMed |
description | Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly understood. Here, we present structures of two such accessory factors from the oncogenic herpesviruses Kaposi’s sarcoma-associated herpesvirus (KSHV; ORF68) and Epstein–Barr virus (EBV; BFLF1). These homologous proteins form highly similar homopentameric rings with a positively charged central channel that binds double-stranded DNA. Mutation of individual positively charged residues within but not outside the channel ablates DNA binding, and in the context of KSHV infection, these mutants fail to package the viral genome or produce progeny virions. Thus, we propose a model in which ORF68 facilitates the transfer of newly replicated viral genomes to the packaging motor. |
format | Online Article Text |
id | pubmed-7889075 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-78890752021-02-18 A pentameric protein ring with novel architecture is required for herpesviral packaging Didychuk, Allison L Gates, Stephanie N Gardner, Matthew R Strong, Lisa M Martin, Andreas Glaunsinger, Britt A eLife Microbiology and Infectious Disease Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly understood. Here, we present structures of two such accessory factors from the oncogenic herpesviruses Kaposi’s sarcoma-associated herpesvirus (KSHV; ORF68) and Epstein–Barr virus (EBV; BFLF1). These homologous proteins form highly similar homopentameric rings with a positively charged central channel that binds double-stranded DNA. Mutation of individual positively charged residues within but not outside the channel ablates DNA binding, and in the context of KSHV infection, these mutants fail to package the viral genome or produce progeny virions. Thus, we propose a model in which ORF68 facilitates the transfer of newly replicated viral genomes to the packaging motor. eLife Sciences Publications, Ltd 2021-02-08 /pmc/articles/PMC7889075/ /pubmed/33554858 http://dx.doi.org/10.7554/eLife.62261 Text en © 2021, Didychuk et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Didychuk, Allison L Gates, Stephanie N Gardner, Matthew R Strong, Lisa M Martin, Andreas Glaunsinger, Britt A A pentameric protein ring with novel architecture is required for herpesviral packaging |
title | A pentameric protein ring with novel architecture is required for herpesviral packaging |
title_full | A pentameric protein ring with novel architecture is required for herpesviral packaging |
title_fullStr | A pentameric protein ring with novel architecture is required for herpesviral packaging |
title_full_unstemmed | A pentameric protein ring with novel architecture is required for herpesviral packaging |
title_short | A pentameric protein ring with novel architecture is required for herpesviral packaging |
title_sort | pentameric protein ring with novel architecture is required for herpesviral packaging |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889075/ https://www.ncbi.nlm.nih.gov/pubmed/33554858 http://dx.doi.org/10.7554/eLife.62261 |
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