Cargando…
YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
The foodborne pathogen Listeria monocytogenes relies on its ability to fine-tune the expression of virulence factors and stress regulators in response to rapidly changing environments. Here, we reveal that YjbH, a putative thioredoxin family oxidoreductase, plays a pivotal role in bacterial adaption...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889195/ https://www.ncbi.nlm.nih.gov/pubmed/33573432 http://dx.doi.org/10.1080/19490976.2021.1884517 |
_version_ | 1783652260426809344 |
---|---|
author | Cheng, Changyong Han, Xiao Xu, Jiali Sun, Jing Li, Kang Han, Yue Chen, Mianmian Song, Houhui |
author_facet | Cheng, Changyong Han, Xiao Xu, Jiali Sun, Jing Li, Kang Han, Yue Chen, Mianmian Song, Houhui |
author_sort | Cheng, Changyong |
collection | PubMed |
description | The foodborne pathogen Listeria monocytogenes relies on its ability to fine-tune the expression of virulence factors and stress regulators in response to rapidly changing environments. Here, we reveal that YjbH, a putative thioredoxin family oxidoreductase, plays a pivotal role in bacterial adaption to oxidative stress and host infection. YjbH directly interacts with SpxA1, an ArsC family oxidative stress response regulator, and the deletion of YjbH compromised the oxidative stress tolerance of L. monocytogenes. Also, YjbH is required for the bacterial spread in host cells and proliferation in mouse organs, thereby contributing to virulence. Transcriptomic analysis of strains treated with Cd(2+) revealed that most virulence genes and phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) genes were significantly downregulated in the absence of YjbH. However, YjbH inhibits PrfA expression when bacteria were grown in the media, suggesting that YjbH participates in regulating the virulence genes via a complicated regulatory network involving PrfA and PTS. Collectively, these findings provide a valuable model for clarifying the roles of thioredoxins from foodborne pathogens regarding improving survival in the external environment and, more importantly, successfully establishing infection within the host. |
format | Online Article Text |
id | pubmed-7889195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78891952021-02-23 YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes Cheng, Changyong Han, Xiao Xu, Jiali Sun, Jing Li, Kang Han, Yue Chen, Mianmian Song, Houhui Gut Microbes Research Paper The foodborne pathogen Listeria monocytogenes relies on its ability to fine-tune the expression of virulence factors and stress regulators in response to rapidly changing environments. Here, we reveal that YjbH, a putative thioredoxin family oxidoreductase, plays a pivotal role in bacterial adaption to oxidative stress and host infection. YjbH directly interacts with SpxA1, an ArsC family oxidative stress response regulator, and the deletion of YjbH compromised the oxidative stress tolerance of L. monocytogenes. Also, YjbH is required for the bacterial spread in host cells and proliferation in mouse organs, thereby contributing to virulence. Transcriptomic analysis of strains treated with Cd(2+) revealed that most virulence genes and phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) genes were significantly downregulated in the absence of YjbH. However, YjbH inhibits PrfA expression when bacteria were grown in the media, suggesting that YjbH participates in regulating the virulence genes via a complicated regulatory network involving PrfA and PTS. Collectively, these findings provide a valuable model for clarifying the roles of thioredoxins from foodborne pathogens regarding improving survival in the external environment and, more importantly, successfully establishing infection within the host. Taylor & Francis 2021-02-12 /pmc/articles/PMC7889195/ /pubmed/33573432 http://dx.doi.org/10.1080/19490976.2021.1884517 Text en © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Cheng, Changyong Han, Xiao Xu, Jiali Sun, Jing Li, Kang Han, Yue Chen, Mianmian Song, Houhui YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes |
title | YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes |
title_full | YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes |
title_fullStr | YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes |
title_full_unstemmed | YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes |
title_short | YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes |
title_sort | yjbh mediates the oxidative stress response and infection by regulating spxa1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (pts) in listeria monocytogenes |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889195/ https://www.ncbi.nlm.nih.gov/pubmed/33573432 http://dx.doi.org/10.1080/19490976.2021.1884517 |
work_keys_str_mv | AT chengchangyong yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes AT hanxiao yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes AT xujiali yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes AT sunjing yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes AT likang yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes AT hanyue yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes AT chenmianmian yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes AT songhouhui yjbhmediatestheoxidativestressresponseandinfectionbyregulatingspxa1andthephosphoenolpyruvatecarbohydratephosphotransferasesystemptsinlisteriamonocytogenes |