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Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA
Beauveria bassiana, known for its entomopathogenic characteristics, is the most widely used biocontrol agent against many insect pests and may also be active against soil-borne pathogens. It inhabits the surfaces or inner tissues of various plant species without causing any visible signs or symptoms...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889226/ https://www.ncbi.nlm.nih.gov/pubmed/33628607 http://dx.doi.org/10.1080/21501203.2019.1707723 |
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author | Nishi, Oumi Sushida, Hirotoshi Higashi, Yumiko Iida, Yuichiro |
author_facet | Nishi, Oumi Sushida, Hirotoshi Higashi, Yumiko Iida, Yuichiro |
author_sort | Nishi, Oumi |
collection | PubMed |
description | Beauveria bassiana, known for its entomopathogenic characteristics, is the most widely used biocontrol agent against many insect pests and may also be active against soil-borne pathogens. It inhabits the surfaces or inner tissues of various plant species without causing any visible signs or symptoms. Here we show that B. bassiana strain GHA, the active ingredient of a commercial microbial insecticide, colonises tomato plants. GHA grew on intact leaf surfaces of tomato in high humidity, but never entered stomata. Viable hyphae and conidia were detected, and the population on inoculated leaves significantly increased until 14 days after inoculation. On tomato leaves, GHA conidiated normally via conidiophores and phialides, and also via microcycle conidiation (conidiophores and phialides form directly from germ tubes and produce conidia). Hyphae were also detected inside the rachis, even more frequently after plant surfaces were scarified. These results suggested that B. bassiana strain GHA can grow epiphytically and endophytically on tomato plants. |
format | Online Article Text |
id | pubmed-7889226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78892262021-02-23 Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA Nishi, Oumi Sushida, Hirotoshi Higashi, Yumiko Iida, Yuichiro Mycology Research Article Beauveria bassiana, known for its entomopathogenic characteristics, is the most widely used biocontrol agent against many insect pests and may also be active against soil-borne pathogens. It inhabits the surfaces or inner tissues of various plant species without causing any visible signs or symptoms. Here we show that B. bassiana strain GHA, the active ingredient of a commercial microbial insecticide, colonises tomato plants. GHA grew on intact leaf surfaces of tomato in high humidity, but never entered stomata. Viable hyphae and conidia were detected, and the population on inoculated leaves significantly increased until 14 days after inoculation. On tomato leaves, GHA conidiated normally via conidiophores and phialides, and also via microcycle conidiation (conidiophores and phialides form directly from germ tubes and produce conidia). Hyphae were also detected inside the rachis, even more frequently after plant surfaces were scarified. These results suggested that B. bassiana strain GHA can grow epiphytically and endophytically on tomato plants. Taylor & Francis 2020-01-05 /pmc/articles/PMC7889226/ /pubmed/33628607 http://dx.doi.org/10.1080/21501203.2019.1707723 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nishi, Oumi Sushida, Hirotoshi Higashi, Yumiko Iida, Yuichiro Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA |
title | Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA |
title_full | Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA |
title_fullStr | Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA |
title_full_unstemmed | Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA |
title_short | Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA |
title_sort | epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus beauveria bassiana strain gha |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889226/ https://www.ncbi.nlm.nih.gov/pubmed/33628607 http://dx.doi.org/10.1080/21501203.2019.1707723 |
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