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Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands
Secretory granules (SGs) are organelles responsible for regulated exocytosis of biologically active molecules in professional secretory cells. Maturation of SGs is a crucial process in which cargoes of SGs are processed and activated, allowing them to exert their function upon secretion. Nonetheless...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889263/ https://www.ncbi.nlm.nih.gov/pubmed/33628358 http://dx.doi.org/10.1080/19420889.2021.1874663 |
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author | Ma, Cheng-I Jonathan Brill, Julie A. |
author_facet | Ma, Cheng-I Jonathan Brill, Julie A. |
author_sort | Ma, Cheng-I Jonathan |
collection | PubMed |
description | Secretory granules (SGs) are organelles responsible for regulated exocytosis of biologically active molecules in professional secretory cells. Maturation of SGs is a crucial process in which cargoes of SGs are processed and activated, allowing them to exert their function upon secretion. Nonetheless, the intracellular trafficking pathways required for SG maturation are not well defined. We recently performed an RNA interference (RNAi) screen in Drosophila larval salivary glands to identify trafficking components needed for SG maturation. From the screen, we identified several Rab GTPases (Rabs) that affect SG maturation. Expression of constitutively active (CA) and dominant-negative (DN) forms narrowed down the Rabs important for this process to Rab5, Rab9 and Rab11. However, none of these Rabs localizes to the limiting membrane of SGs. In contrast, examination of endogenously YFP-tagged Rabs (YRabs) in larval salivary glands revealed that YRab1 and YRab6 localize to the limiting membrane of immature SGs (iSGs) and SGs. These findings provide new insights into how Rab GTPases contribute to the process of SG maturation. |
format | Online Article Text |
id | pubmed-7889263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78892632021-02-23 Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands Ma, Cheng-I Jonathan Brill, Julie A. Commun Integr Biol Short Communication Secretory granules (SGs) are organelles responsible for regulated exocytosis of biologically active molecules in professional secretory cells. Maturation of SGs is a crucial process in which cargoes of SGs are processed and activated, allowing them to exert their function upon secretion. Nonetheless, the intracellular trafficking pathways required for SG maturation are not well defined. We recently performed an RNA interference (RNAi) screen in Drosophila larval salivary glands to identify trafficking components needed for SG maturation. From the screen, we identified several Rab GTPases (Rabs) that affect SG maturation. Expression of constitutively active (CA) and dominant-negative (DN) forms narrowed down the Rabs important for this process to Rab5, Rab9 and Rab11. However, none of these Rabs localizes to the limiting membrane of SGs. In contrast, examination of endogenously YFP-tagged Rabs (YRabs) in larval salivary glands revealed that YRab1 and YRab6 localize to the limiting membrane of immature SGs (iSGs) and SGs. These findings provide new insights into how Rab GTPases contribute to the process of SG maturation. Taylor & Francis 2021-02-09 /pmc/articles/PMC7889263/ /pubmed/33628358 http://dx.doi.org/10.1080/19420889.2021.1874663 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Ma, Cheng-I Jonathan Brill, Julie A. Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands |
title | Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands |
title_full | Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands |
title_fullStr | Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands |
title_full_unstemmed | Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands |
title_short | Endosomal Rab GTPases regulate secretory granule maturation in Drosophila larval salivary glands |
title_sort | endosomal rab gtpases regulate secretory granule maturation in drosophila larval salivary glands |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889263/ https://www.ncbi.nlm.nih.gov/pubmed/33628358 http://dx.doi.org/10.1080/19420889.2021.1874663 |
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