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UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechanism of how cells sense these signals and reprogram the methylation landscape is poorly understood. H...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889724/ https://www.ncbi.nlm.nih.gov/pubmed/33495557 http://dx.doi.org/10.1038/s41477-020-00843-4 |
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author | Jiang, Jianjun Liu, Jie Sanders, Dean Qian, Shuiming Ren, Wendan Song, Jikui Liu, Fengquan Zhong, Xuehua |
author_facet | Jiang, Jianjun Liu, Jie Sanders, Dean Qian, Shuiming Ren, Wendan Song, Jikui Liu, Fengquan Zhong, Xuehua |
author_sort | Jiang, Jianjun |
collection | PubMed |
description | DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechanism of how cells sense these signals and reprogram the methylation landscape is poorly understood. Here, we uncovered a novel connection between ultraviolet B (UVB) signaling and DNA methylation involving UVB photoreceptor (UVR8) and a de novo DNA methyltransferase (DRM2) in Arabidopsis. We demonstrated that UVB acts through UVR8 to inhibit DRM2-mediated DNA methylation and transcriptional de-repression. Interestingly, DNA transposons with high DNA methylation are more sensitive to UVB irradiation. Mechanistically, UVR8 interacts with and negatively regulates DRM2 by preventing its chromatin association and inhibiting the methyltransferase activity. Collectively, this study identifies UVB as a potent inhibitor of DNA methylation and provides mechanistic insights into how signaling transduction cascades intertwine with chromatin to guide genome functions. |
format | Online Article Text |
id | pubmed-7889724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-78897242021-07-25 UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis Jiang, Jianjun Liu, Jie Sanders, Dean Qian, Shuiming Ren, Wendan Song, Jikui Liu, Fengquan Zhong, Xuehua Nat Plants Article DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechanism of how cells sense these signals and reprogram the methylation landscape is poorly understood. Here, we uncovered a novel connection between ultraviolet B (UVB) signaling and DNA methylation involving UVB photoreceptor (UVR8) and a de novo DNA methyltransferase (DRM2) in Arabidopsis. We demonstrated that UVB acts through UVR8 to inhibit DRM2-mediated DNA methylation and transcriptional de-repression. Interestingly, DNA transposons with high DNA methylation are more sensitive to UVB irradiation. Mechanistically, UVR8 interacts with and negatively regulates DRM2 by preventing its chromatin association and inhibiting the methyltransferase activity. Collectively, this study identifies UVB as a potent inhibitor of DNA methylation and provides mechanistic insights into how signaling transduction cascades intertwine with chromatin to guide genome functions. 2021-01-25 2021-02 /pmc/articles/PMC7889724/ /pubmed/33495557 http://dx.doi.org/10.1038/s41477-020-00843-4 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Jiang, Jianjun Liu, Jie Sanders, Dean Qian, Shuiming Ren, Wendan Song, Jikui Liu, Fengquan Zhong, Xuehua UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis |
title | UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis |
title_full | UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis |
title_fullStr | UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis |
title_full_unstemmed | UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis |
title_short | UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis |
title_sort | uvr8 interacts with de novo dna methyltransferase and suppresses dna methylation in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889724/ https://www.ncbi.nlm.nih.gov/pubmed/33495557 http://dx.doi.org/10.1038/s41477-020-00843-4 |
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