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UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis

DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechanism of how cells sense these signals and reprogram the methylation landscape is poorly understood. H...

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Detalles Bibliográficos
Autores principales: Jiang, Jianjun, Liu, Jie, Sanders, Dean, Qian, Shuiming, Ren, Wendan, Song, Jikui, Liu, Fengquan, Zhong, Xuehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889724/
https://www.ncbi.nlm.nih.gov/pubmed/33495557
http://dx.doi.org/10.1038/s41477-020-00843-4
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author Jiang, Jianjun
Liu, Jie
Sanders, Dean
Qian, Shuiming
Ren, Wendan
Song, Jikui
Liu, Fengquan
Zhong, Xuehua
author_facet Jiang, Jianjun
Liu, Jie
Sanders, Dean
Qian, Shuiming
Ren, Wendan
Song, Jikui
Liu, Fengquan
Zhong, Xuehua
author_sort Jiang, Jianjun
collection PubMed
description DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechanism of how cells sense these signals and reprogram the methylation landscape is poorly understood. Here, we uncovered a novel connection between ultraviolet B (UVB) signaling and DNA methylation involving UVB photoreceptor (UVR8) and a de novo DNA methyltransferase (DRM2) in Arabidopsis. We demonstrated that UVB acts through UVR8 to inhibit DRM2-mediated DNA methylation and transcriptional de-repression. Interestingly, DNA transposons with high DNA methylation are more sensitive to UVB irradiation. Mechanistically, UVR8 interacts with and negatively regulates DRM2 by preventing its chromatin association and inhibiting the methyltransferase activity. Collectively, this study identifies UVB as a potent inhibitor of DNA methylation and provides mechanistic insights into how signaling transduction cascades intertwine with chromatin to guide genome functions.
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spelling pubmed-78897242021-07-25 UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis Jiang, Jianjun Liu, Jie Sanders, Dean Qian, Shuiming Ren, Wendan Song, Jikui Liu, Fengquan Zhong, Xuehua Nat Plants Article DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechanism of how cells sense these signals and reprogram the methylation landscape is poorly understood. Here, we uncovered a novel connection between ultraviolet B (UVB) signaling and DNA methylation involving UVB photoreceptor (UVR8) and a de novo DNA methyltransferase (DRM2) in Arabidopsis. We demonstrated that UVB acts through UVR8 to inhibit DRM2-mediated DNA methylation and transcriptional de-repression. Interestingly, DNA transposons with high DNA methylation are more sensitive to UVB irradiation. Mechanistically, UVR8 interacts with and negatively regulates DRM2 by preventing its chromatin association and inhibiting the methyltransferase activity. Collectively, this study identifies UVB as a potent inhibitor of DNA methylation and provides mechanistic insights into how signaling transduction cascades intertwine with chromatin to guide genome functions. 2021-01-25 2021-02 /pmc/articles/PMC7889724/ /pubmed/33495557 http://dx.doi.org/10.1038/s41477-020-00843-4 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Jiang, Jianjun
Liu, Jie
Sanders, Dean
Qian, Shuiming
Ren, Wendan
Song, Jikui
Liu, Fengquan
Zhong, Xuehua
UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
title UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
title_full UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
title_fullStr UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
title_full_unstemmed UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
title_short UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
title_sort uvr8 interacts with de novo dna methyltransferase and suppresses dna methylation in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889724/
https://www.ncbi.nlm.nih.gov/pubmed/33495557
http://dx.doi.org/10.1038/s41477-020-00843-4
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