Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing

Simultaneous expression of multiplex guide RNAs (gRNAs) is valuable for knockout of multiple genes and also for effective disruption of a gene by introducing multiple deletions. We developed a method of Tetraplex-guide Tandem for construction of cosmids containing four and eight multiplex gRNA-expre...

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Detalles Bibliográficos
Autores principales: Nakanishi, Tomoko, Maekawa, Aya, Suzuki, Mariko, Tabata, Hirotaka, Sato, Kumiko, Mori, Mai, Saito, Izumu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889857/
https://www.ncbi.nlm.nih.gov/pubmed/33597562
http://dx.doi.org/10.1038/s41598-021-83259-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889857/
https://www.ncbi.nlm.nih.gov/pubmed/33597562
http://dx.doi.org/10.1038/s41598-021-83259-0

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