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Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons

Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a proto...

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Detalles Bibliográficos
Autores principales: Moro, Alessandro, Hoogstraaten, Rein I., Persoon, Claudia M., Verhage, Matthijs, Toonen, Ruud F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890040/
https://www.ncbi.nlm.nih.gov/pubmed/33659902
http://dx.doi.org/10.1016/j.xpro.2021.100325
Descripción
Sumario:Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechanisms and is easily adapted to investigate fusion principles of other secretory organelles. For complete details on the use and execution of this protocol, please refer to Persoon et al. (2019).