Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations

Here, we describe a generic protocol for monitoring protein-RNA interaction using a cleavable GFP fusion of a recombinant RNA-binding protein. We detail each expression and purification step, including high salt and heparin column for contaminant RNA removal. After the assembly of RNA into the ribon...

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Detalles Bibliográficos
Autores principales: Gao, Yunrong, Cao, Dongdong, Pawnikar, Shristi, Akhter, Sana, Miao, Yinglong, Liang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890042/
https://www.ncbi.nlm.nih.gov/pubmed/33659898
http://dx.doi.org/10.1016/j.xpro.2021.100315

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