Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells

We describe two differentiation protocols to derive sensory spinal interneurons (INs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In protocol 1, we use retinoic acid (RA) to induce pain, itch, and heat mediating dI4/dI6 interneurons, and in protocol 2, RA with...

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Detalles Bibliográficos
Autores principales: Gupta, Sandeep, Yamauchi, Ken, Novitch, Bennett G., Butler, Samantha J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890043/
https://www.ncbi.nlm.nih.gov/pubmed/33659900
http://dx.doi.org/10.1016/j.xpro.2021.100319
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author Gupta, Sandeep
Yamauchi, Ken
Novitch, Bennett G.
Butler, Samantha J.
author_facet Gupta, Sandeep
Yamauchi, Ken
Novitch, Bennett G.
Butler, Samantha J.
author_sort Gupta, Sandeep
collection PubMed
description We describe two differentiation protocols to derive sensory spinal interneurons (INs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In protocol 1, we use retinoic acid (RA) to induce pain, itch, and heat mediating dI4/dI6 interneurons, and in protocol 2, RA with bone morphogenetic protein 4 (RA+BMP4) is used to induce proprioceptive dI1s and mechanosensory dI3s in hPSC cultures. These protocols provide an important step toward developing therapies for regaining sensation in spinal cord injury patients. For complete details on the use and execution of this protocol, please refer to Gupta et al. (2018).
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spelling pubmed-78900432021-03-02 Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells Gupta, Sandeep Yamauchi, Ken Novitch, Bennett G. Butler, Samantha J. STAR Protoc Protocol We describe two differentiation protocols to derive sensory spinal interneurons (INs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In protocol 1, we use retinoic acid (RA) to induce pain, itch, and heat mediating dI4/dI6 interneurons, and in protocol 2, RA with bone morphogenetic protein 4 (RA+BMP4) is used to induce proprioceptive dI1s and mechanosensory dI3s in hPSC cultures. These protocols provide an important step toward developing therapies for regaining sensation in spinal cord injury patients. For complete details on the use and execution of this protocol, please refer to Gupta et al. (2018). Elsevier 2021-02-03 /pmc/articles/PMC7890043/ /pubmed/33659900 http://dx.doi.org/10.1016/j.xpro.2021.100319 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gupta, Sandeep
Yamauchi, Ken
Novitch, Bennett G.
Butler, Samantha J.
Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
title Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
title_full Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
title_fullStr Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
title_full_unstemmed Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
title_short Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
title_sort derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890043/
https://www.ncbi.nlm.nih.gov/pubmed/33659900
http://dx.doi.org/10.1016/j.xpro.2021.100319
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