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Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells

We describe two differentiation protocols to derive sensory spinal interneurons (INs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In protocol 1, we use retinoic acid (RA) to induce pain, itch, and heat mediating dI4/dI6 interneurons, and in protocol 2, RA with...

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Detalles Bibliográficos
Autores principales:	Gupta, Sandeep, Yamauchi, Ken, Novitch, Bennett G., Butler, Samantha J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890043/ https://www.ncbi.nlm.nih.gov/pubmed/33659900 http://dx.doi.org/10.1016/j.xpro.2021.100319

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