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The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells

Autotransporter proteins are widely present in Gram-negative bacteria. They play a pivotal role in processes related to bacterial pathogenesis, including adhesion, invasion, colonization, biofilm formation, and cellular toxicity. Bioinformatics analysis revealed that Orientia tsutsugamushi, the caus...

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Autores principales: Nguyen, Yen Thi Hai, Kim, Chaewon, Kim, Yuri, Jeon, Kyeongseok, Kim, Hong-il, Ha, Na-Young, Cho, Nam-Hyuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890071/
https://www.ncbi.nlm.nih.gov/pubmed/33613493
http://dx.doi.org/10.3389/fmicb.2021.626298
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author Nguyen, Yen Thi Hai
Kim, Chaewon
Kim, Yuri
Jeon, Kyeongseok
Kim, Hong-il
Ha, Na-Young
Cho, Nam-Hyuk
author_facet Nguyen, Yen Thi Hai
Kim, Chaewon
Kim, Yuri
Jeon, Kyeongseok
Kim, Hong-il
Ha, Na-Young
Cho, Nam-Hyuk
author_sort Nguyen, Yen Thi Hai
collection PubMed
description Autotransporter proteins are widely present in Gram-negative bacteria. They play a pivotal role in processes related to bacterial pathogenesis, including adhesion, invasion, colonization, biofilm formation, and cellular toxicity. Bioinformatics analysis revealed that Orientia tsutsugamushi, the causative agent of scrub typhus, encodes six different autotransporter genes (scaA–scaF). Although four of these genes (scaA, scaC, scaD, and scaE) are present in diverse strains, scaB and scaF have been detected in only a limited number of strains. Previous studies have demonstrated that ScaA and ScaC are involved in the adherence of host cells. However, the putative function of other O. tsutsugamushi Sca proteins has not been studied yet. In this study, we show that scaB is transcribed and expressed on the surface of O. tsutsugamushi Boryong strain. Using a heterologous Escherichia coli expression system, we demonstrated that ScaB-expressing E. coli can successfully mediate adherence to and invasion into non-phagocytic cells, including epithelial and endothelial cells. In addition, pretreatment with a recombinant ScaB polypeptide inhibits the entry of O. tsutsugamushi into cultured mammalian cells. Finally, we also identified the scaB gene in the Kuroki and TA686 strains and observed high levels of sequence variation in the passenger domains. Here, we propose that the ScaB protein of O. tsutsugamushi can mediate both adhesion to and invasion into host cells in the absence of other O. tsutsugamushi genes and may play important roles in bacterial pathogenesis.
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spelling pubmed-78900712021-02-19 The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells Nguyen, Yen Thi Hai Kim, Chaewon Kim, Yuri Jeon, Kyeongseok Kim, Hong-il Ha, Na-Young Cho, Nam-Hyuk Front Microbiol Microbiology Autotransporter proteins are widely present in Gram-negative bacteria. They play a pivotal role in processes related to bacterial pathogenesis, including adhesion, invasion, colonization, biofilm formation, and cellular toxicity. Bioinformatics analysis revealed that Orientia tsutsugamushi, the causative agent of scrub typhus, encodes six different autotransporter genes (scaA–scaF). Although four of these genes (scaA, scaC, scaD, and scaE) are present in diverse strains, scaB and scaF have been detected in only a limited number of strains. Previous studies have demonstrated that ScaA and ScaC are involved in the adherence of host cells. However, the putative function of other O. tsutsugamushi Sca proteins has not been studied yet. In this study, we show that scaB is transcribed and expressed on the surface of O. tsutsugamushi Boryong strain. Using a heterologous Escherichia coli expression system, we demonstrated that ScaB-expressing E. coli can successfully mediate adherence to and invasion into non-phagocytic cells, including epithelial and endothelial cells. In addition, pretreatment with a recombinant ScaB polypeptide inhibits the entry of O. tsutsugamushi into cultured mammalian cells. Finally, we also identified the scaB gene in the Kuroki and TA686 strains and observed high levels of sequence variation in the passenger domains. Here, we propose that the ScaB protein of O. tsutsugamushi can mediate both adhesion to and invasion into host cells in the absence of other O. tsutsugamushi genes and may play important roles in bacterial pathogenesis. Frontiers Media S.A. 2021-02-04 /pmc/articles/PMC7890071/ /pubmed/33613493 http://dx.doi.org/10.3389/fmicb.2021.626298 Text en Copyright © 2021 Nguyen, Kim, Kim, Jeon, Kim, Ha and Cho. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Nguyen, Yen Thi Hai
Kim, Chaewon
Kim, Yuri
Jeon, Kyeongseok
Kim, Hong-il
Ha, Na-Young
Cho, Nam-Hyuk
The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells
title The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells
title_full The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells
title_fullStr The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells
title_full_unstemmed The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells
title_short The Orientia tsutsugamushi ScaB Autotransporter Protein Is Required for Adhesion and Invasion of Mammalian Cells
title_sort orientia tsutsugamushi scab autotransporter protein is required for adhesion and invasion of mammalian cells
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890071/
https://www.ncbi.nlm.nih.gov/pubmed/33613493
http://dx.doi.org/10.3389/fmicb.2021.626298
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