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Morphological analyses of nephrin expression in progressive glomerulonephropathy of common marmosets
In this study, we focused on nephrin, one of the key molecules within the slit diaphragm of podocytes, as although there have been reports on its expression in humans and rats, their presence in common marmosets has not been reported. We investigated nephrin expression and changes in glomeruli, depe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Toxicologic Pathology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890167/ https://www.ncbi.nlm.nih.gov/pubmed/33627947 http://dx.doi.org/10.1293/tox.2020-0056 |
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author | Yamada, Naoaki Doi, Takuya Sato, Junko Inoue, Takeshi Tsuchitani, Minoru Kobayashi, Yoshiyasu |
author_facet | Yamada, Naoaki Doi, Takuya Sato, Junko Inoue, Takeshi Tsuchitani, Minoru Kobayashi, Yoshiyasu |
author_sort | Yamada, Naoaki |
collection | PubMed |
description | In this study, we focused on nephrin, one of the key molecules within the slit diaphragm of podocytes, as although there have been reports on its expression in humans and rats, their presence in common marmosets has not been reported. We investigated nephrin expression and changes in glomeruli, depending on the development of spontaneous progressive glomerulonephropathy in common marmosets. Nineteen common marmosets at two to ten years of age were evaluated. The kidney was examined by microscopy with hematoxylin and eosin and immunohistochemical staining for nephrin. The lesions were classified into three grades according to a renal lesion grading system reported previously. The nephrin-positive area was measured by morphometric analysis, and the nephrin-positive ratio was calculated. Nephrin expression was observed along the glomerular capillary loop in a continuous linear pattern in renal lesion grades 0 to 2 and either discontinuous linear or coarse granular pattern in grade 3. Nephrin expression tended to decrease significantly depending on the grade of renal lesions. Alteration in nephrin expression has been suggested to play an important role in the progression of renal lesions. |
format | Online Article Text |
id | pubmed-7890167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Japanese Society of Toxicologic Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-78901672021-02-23 Morphological analyses of nephrin expression in progressive glomerulonephropathy of common marmosets Yamada, Naoaki Doi, Takuya Sato, Junko Inoue, Takeshi Tsuchitani, Minoru Kobayashi, Yoshiyasu J Toxicol Pathol Original Article In this study, we focused on nephrin, one of the key molecules within the slit diaphragm of podocytes, as although there have been reports on its expression in humans and rats, their presence in common marmosets has not been reported. We investigated nephrin expression and changes in glomeruli, depending on the development of spontaneous progressive glomerulonephropathy in common marmosets. Nineteen common marmosets at two to ten years of age were evaluated. The kidney was examined by microscopy with hematoxylin and eosin and immunohistochemical staining for nephrin. The lesions were classified into three grades according to a renal lesion grading system reported previously. The nephrin-positive area was measured by morphometric analysis, and the nephrin-positive ratio was calculated. Nephrin expression was observed along the glomerular capillary loop in a continuous linear pattern in renal lesion grades 0 to 2 and either discontinuous linear or coarse granular pattern in grade 3. Nephrin expression tended to decrease significantly depending on the grade of renal lesions. Alteration in nephrin expression has been suggested to play an important role in the progression of renal lesions. Japanese Society of Toxicologic Pathology 2020-11-21 2021-01 /pmc/articles/PMC7890167/ /pubmed/33627947 http://dx.doi.org/10.1293/tox.2020-0056 Text en ©2021 The Japanese Society of Toxicologic Pathology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Yamada, Naoaki Doi, Takuya Sato, Junko Inoue, Takeshi Tsuchitani, Minoru Kobayashi, Yoshiyasu Morphological analyses of nephrin expression in progressive glomerulonephropathy of common marmosets |
title | Morphological analyses of nephrin expression in progressive
glomerulonephropathy of common marmosets |
title_full | Morphological analyses of nephrin expression in progressive
glomerulonephropathy of common marmosets |
title_fullStr | Morphological analyses of nephrin expression in progressive
glomerulonephropathy of common marmosets |
title_full_unstemmed | Morphological analyses of nephrin expression in progressive
glomerulonephropathy of common marmosets |
title_short | Morphological analyses of nephrin expression in progressive
glomerulonephropathy of common marmosets |
title_sort | morphological analyses of nephrin expression in progressive
glomerulonephropathy of common marmosets |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890167/ https://www.ncbi.nlm.nih.gov/pubmed/33627947 http://dx.doi.org/10.1293/tox.2020-0056 |
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