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Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats

Occupational exposure to nickel oxide (NiO) is an important cause of respiratory tract cancer. Toxicity is known to be associated with the dissociated component, i.e. nickel (II) ions. To address the relationship between physicochemical properties, including solubility in artificial lysosomal fluid,...

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Autores principales: Kobayashi, Toshio, Oshima, Yutaka, Tsubokura, Yasuhiro, Muroi, Takako, Ajimi, Shozo, Nakai, Makoto, Kawaguchi, Kenji, Sasaki, Takeshi, Shinohara, Naohide, Imatanaka, Nobuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Toxicologic Pathology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890174/
https://www.ncbi.nlm.nih.gov/pubmed/33627944
http://dx.doi.org/10.1293/tox.2020-0066
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author Kobayashi, Toshio
Oshima, Yutaka
Tsubokura, Yasuhiro
Muroi, Takako
Ajimi, Shozo
Nakai, Makoto
Kawaguchi, Kenji
Sasaki, Takeshi
Shinohara, Naohide
Imatanaka, Nobuya
author_facet Kobayashi, Toshio
Oshima, Yutaka
Tsubokura, Yasuhiro
Muroi, Takako
Ajimi, Shozo
Nakai, Makoto
Kawaguchi, Kenji
Sasaki, Takeshi
Shinohara, Naohide
Imatanaka, Nobuya
author_sort Kobayashi, Toshio
collection PubMed
description Occupational exposure to nickel oxide (NiO) is an important cause of respiratory tract cancer. Toxicity is known to be associated with the dissociated component, i.e. nickel (II) ions. To address the relationship between physicochemical properties, including solubility in artificial lysosomal fluid, of NiO and time-course changes in the pulmonary response, we conducted an intratracheal instillation study in male Fischer rats using four different well-characterized NiO products, US3352 (NiO A), NovaWireNi01 (NiO B), I small particle (NiO C), and 637130 (NiO D). The NiOs were suspended in purified water and instilled once intratracheally into male F344 rats (12 weeks old) at 0 (vehicle control), 0.67, 2, and 6 mg/kg body weight. The animals were euthanized on days 3, 28, or 91 after instillation, and blood analysis, bronchoalveolar lavage fluid (BALF) testing, and histopathological examination were performed. The most soluble product, NiO B, caused the most severe systemic toxicity, leading to a high mortality rate, but the response was transient and surviving animals recovered. The second-most-soluble material, NiO D, and the third, NiO A, caused evident pulmonary inflammation, and the responses persisted for at least 91 days with collagen proliferation. In contrast, NiO C induced barely detectable inflammation in the BALF examination, and no marked changes were noted on histopathology. These results indicate that the early phase toxic potential of NiO products, but not the persistence of pulmonary inflammation, is associated with their solubility.
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spelling pubmed-78901742021-02-23 Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats Kobayashi, Toshio Oshima, Yutaka Tsubokura, Yasuhiro Muroi, Takako Ajimi, Shozo Nakai, Makoto Kawaguchi, Kenji Sasaki, Takeshi Shinohara, Naohide Imatanaka, Nobuya J Toxicol Pathol Original Article Occupational exposure to nickel oxide (NiO) is an important cause of respiratory tract cancer. Toxicity is known to be associated with the dissociated component, i.e. nickel (II) ions. To address the relationship between physicochemical properties, including solubility in artificial lysosomal fluid, of NiO and time-course changes in the pulmonary response, we conducted an intratracheal instillation study in male Fischer rats using four different well-characterized NiO products, US3352 (NiO A), NovaWireNi01 (NiO B), I small particle (NiO C), and 637130 (NiO D). The NiOs were suspended in purified water and instilled once intratracheally into male F344 rats (12 weeks old) at 0 (vehicle control), 0.67, 2, and 6 mg/kg body weight. The animals were euthanized on days 3, 28, or 91 after instillation, and blood analysis, bronchoalveolar lavage fluid (BALF) testing, and histopathological examination were performed. The most soluble product, NiO B, caused the most severe systemic toxicity, leading to a high mortality rate, but the response was transient and surviving animals recovered. The second-most-soluble material, NiO D, and the third, NiO A, caused evident pulmonary inflammation, and the responses persisted for at least 91 days with collagen proliferation. In contrast, NiO C induced barely detectable inflammation in the BALF examination, and no marked changes were noted on histopathology. These results indicate that the early phase toxic potential of NiO products, but not the persistence of pulmonary inflammation, is associated with their solubility. Japanese Society of Toxicologic Pathology 2020-11-12 2021-01 /pmc/articles/PMC7890174/ /pubmed/33627944 http://dx.doi.org/10.1293/tox.2020-0066 Text en ©2021 The Japanese Society of Toxicologic Pathology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Kobayashi, Toshio
Oshima, Yutaka
Tsubokura, Yasuhiro
Muroi, Takako
Ajimi, Shozo
Nakai, Makoto
Kawaguchi, Kenji
Sasaki, Takeshi
Shinohara, Naohide
Imatanaka, Nobuya
Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats
title Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats
title_full Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats
title_fullStr Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats
title_full_unstemmed Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats
title_short Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats
title_sort time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male fischer rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890174/
https://www.ncbi.nlm.nih.gov/pubmed/33627944
http://dx.doi.org/10.1293/tox.2020-0066
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