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Nuclear RNA purification by flow cytometry to study nuclear processes in plants
The nature of plant tissues has continuously hampered understanding of the spatio-temporal and subcellular distribution of RNA-guided processes. Here, we describe a universal protocol based on Arabidopsis to investigate subcellular RNA distribution from virtually any plant species using flow cytomet...
Autores principales: | Moro, Belén, Kisielow, Malgorzata, Borrero, Veronica Barragan, Bouet, Antoine, Brosnan, Christopher A., Bologna, Nicolás G. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890302/ https://www.ncbi.nlm.nih.gov/pubmed/33659901 http://dx.doi.org/10.1016/j.xpro.2021.100320 |
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