LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis

Objective: To clarify the role of LINC00665 in ovarian cancer (OC) progression and the possible mechanism. Methods: LINC00665 levels in OC tissues and cell lines were detected by qRT-PCR. The correlation between LINC00665 and clinicopathologic characteristics of OC patients was assessed. Biological...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Dan, Song, Qingxia, Liu, Ying, Chen, Wansu, LU, Lijuan, Xu, Min, Fang, Xiaohui, Zhao, Wenjie, Zhou, Huifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890326/
https://www.ncbi.nlm.nih.gov/pubmed/33613764
http://dx.doi.org/10.7150/jca.51457
_version_ 1783652492537495552
author Xu, Dan
Song, Qingxia
Liu, Ying
Chen, Wansu
LU, Lijuan
Xu, Min
Fang, Xiaohui
Zhao, Wenjie
Zhou, Huifang
author_facet Xu, Dan
Song, Qingxia
Liu, Ying
Chen, Wansu
LU, Lijuan
Xu, Min
Fang, Xiaohui
Zhao, Wenjie
Zhou, Huifang
author_sort Xu, Dan
collection PubMed
description Objective: To clarify the role of LINC00665 in ovarian cancer (OC) progression and the possible mechanism. Methods: LINC00665 levels in OC tissues and cell lines were detected by qRT-PCR. The correlation between LINC00665 and clinicopathologic characteristics of OC patients was assessed. Biological functions of OC cell phenotypes influenced by LINC00665 were examined by CCK-8, colony formation and Transwell assay. Dual-luciferase reporter assay and RIP assay were conducted to verify the interaction between LINC00665 and its downstream target. Results: LINC00665 was upregulated in OC and linked to poor prognosis. Knockdown of LINC00665 blocked malignant proliferative, migratory and invasive functions of OC cells. By competitively binding miRNA-34a-5p, LINC00665 abolished the inhibitory effect of miR-34a-3p on its downstream gene E2F3, thus promoting OC progression. Conclusion: LINC00665/miRNA-34a-5p/E2F3 axis is involved in OC progression, providing novel insights into the clinical treatment of OC.
format Online
Article
Text
id pubmed-7890326
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-78903262021-02-18 LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis Xu, Dan Song, Qingxia Liu, Ying Chen, Wansu LU, Lijuan Xu, Min Fang, Xiaohui Zhao, Wenjie Zhou, Huifang J Cancer Research Paper Objective: To clarify the role of LINC00665 in ovarian cancer (OC) progression and the possible mechanism. Methods: LINC00665 levels in OC tissues and cell lines were detected by qRT-PCR. The correlation between LINC00665 and clinicopathologic characteristics of OC patients was assessed. Biological functions of OC cell phenotypes influenced by LINC00665 were examined by CCK-8, colony formation and Transwell assay. Dual-luciferase reporter assay and RIP assay were conducted to verify the interaction between LINC00665 and its downstream target. Results: LINC00665 was upregulated in OC and linked to poor prognosis. Knockdown of LINC00665 blocked malignant proliferative, migratory and invasive functions of OC cells. By competitively binding miRNA-34a-5p, LINC00665 abolished the inhibitory effect of miR-34a-3p on its downstream gene E2F3, thus promoting OC progression. Conclusion: LINC00665/miRNA-34a-5p/E2F3 axis is involved in OC progression, providing novel insights into the clinical treatment of OC. Ivyspring International Publisher 2021-01-18 /pmc/articles/PMC7890326/ /pubmed/33613764 http://dx.doi.org/10.7150/jca.51457 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Xu, Dan
Song, Qingxia
Liu, Ying
Chen, Wansu
LU, Lijuan
Xu, Min
Fang, Xiaohui
Zhao, Wenjie
Zhou, Huifang
LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis
title LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis
title_full LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis
title_fullStr LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis
title_full_unstemmed LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis
title_short LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis
title_sort linc00665 promotes ovarian cancer progression through regulating the mirna-34a-5p/e2f3 axis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890326/
https://www.ncbi.nlm.nih.gov/pubmed/33613764
http://dx.doi.org/10.7150/jca.51457
work_keys_str_mv AT xudan linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT songqingxia linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT liuying linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT chenwansu linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT lulijuan linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT xumin linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT fangxiaohui linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT zhaowenjie linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis
AT zhouhuifang linc00665promotesovariancancerprogressionthroughregulatingthemirna34a5pe2f3axis

Ejemplares similares