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G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana

Jasmonic acid (JA) is regarded as an endogenous regulator which plays an important role in regulating plant growth, development and stress response. Using the seedlings of A. thaliana ecotype Col-0 (wild-type, WT), phospholipase Dδ (PLDδ) deficient mutant (pldδ), the G protein α subunit (GPA1) defic...

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Autores principales: Yang, Ning, Zhang, Yue, Chen, Lu, Wang, Wei, Liu, Ruirui, Gao, Run, Zhou, Yaping, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890375/
https://www.ncbi.nlm.nih.gov/pubmed/33659715
http://dx.doi.org/10.1016/j.bbrep.2021.100952
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author Yang, Ning
Zhang, Yue
Chen, Lu
Wang, Wei
Liu, Ruirui
Gao, Run
Zhou, Yaping
Li, Hui
author_facet Yang, Ning
Zhang, Yue
Chen, Lu
Wang, Wei
Liu, Ruirui
Gao, Run
Zhou, Yaping
Li, Hui
author_sort Yang, Ning
collection PubMed
description Jasmonic acid (JA) is regarded as an endogenous regulator which plays an important role in regulating plant growth, development and stress response. Using the seedlings of A. thaliana ecotype Col-0 (wild-type, WT), phospholipase Dδ (PLDδ) deficient mutant (pldδ), the G protein α subunit (GPA1) deficient mutant (gpa1-4), 9-Lipoxygenase (9-LOX) deficient mutants (lox1 and lox5) as materials, the effects of JA responding to osmotic stress and the functions of G protein and PLDδ in this response were investigated. The results showed that GPA1 involved in the regulation of JA to PLDδ under osmotic stress. Both GPA1 and PLDδ participated in the regulation of JA on the seed germination and osmotic tolerance. Exogenous MeJA reduced the EL and MDA in WT, but increased the EL and MDA in gpa1-4 and pldδ, indicating that GPA1 and PLDδ were involved in the protection of JA on the membrane. The genes expression levels, and the activities of PLDδ and LOX1 were significantly induced by osmotic stress. The LOX activity and JA content in pldδ seedings were lower obviously than those in WT, but were markedly increased and were higher than WT after applying phosphatidic acid (PA). These results demonstrated that JA responded to osmotic stress by regulating G protein and PLDδ in A. thaliana. PLDδ was located upstream of 9-LOX and involved in the JA biosynthesis.
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spelling pubmed-78903752021-03-02 G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana Yang, Ning Zhang, Yue Chen, Lu Wang, Wei Liu, Ruirui Gao, Run Zhou, Yaping Li, Hui Biochem Biophys Rep Research Article Jasmonic acid (JA) is regarded as an endogenous regulator which plays an important role in regulating plant growth, development and stress response. Using the seedlings of A. thaliana ecotype Col-0 (wild-type, WT), phospholipase Dδ (PLDδ) deficient mutant (pldδ), the G protein α subunit (GPA1) deficient mutant (gpa1-4), 9-Lipoxygenase (9-LOX) deficient mutants (lox1 and lox5) as materials, the effects of JA responding to osmotic stress and the functions of G protein and PLDδ in this response were investigated. The results showed that GPA1 involved in the regulation of JA to PLDδ under osmotic stress. Both GPA1 and PLDδ participated in the regulation of JA on the seed germination and osmotic tolerance. Exogenous MeJA reduced the EL and MDA in WT, but increased the EL and MDA in gpa1-4 and pldδ, indicating that GPA1 and PLDδ were involved in the protection of JA on the membrane. The genes expression levels, and the activities of PLDδ and LOX1 were significantly induced by osmotic stress. The LOX activity and JA content in pldδ seedings were lower obviously than those in WT, but were markedly increased and were higher than WT after applying phosphatidic acid (PA). These results demonstrated that JA responded to osmotic stress by regulating G protein and PLDδ in A. thaliana. PLDδ was located upstream of 9-LOX and involved in the JA biosynthesis. Elsevier 2021-02-12 /pmc/articles/PMC7890375/ /pubmed/33659715 http://dx.doi.org/10.1016/j.bbrep.2021.100952 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Yang, Ning
Zhang, Yue
Chen, Lu
Wang, Wei
Liu, Ruirui
Gao, Run
Zhou, Yaping
Li, Hui
G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana
title G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana
title_full G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana
title_fullStr G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana
title_full_unstemmed G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana
title_short G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana
title_sort g protein and pldδ are involved in ja to regulate osmotic stress responses in arabidopsis thaliana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890375/
https://www.ncbi.nlm.nih.gov/pubmed/33659715
http://dx.doi.org/10.1016/j.bbrep.2021.100952
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