Cargando…
G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana
Jasmonic acid (JA) is regarded as an endogenous regulator which plays an important role in regulating plant growth, development and stress response. Using the seedlings of A. thaliana ecotype Col-0 (wild-type, WT), phospholipase Dδ (PLDδ) deficient mutant (pldδ), the G protein α subunit (GPA1) defic...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890375/ https://www.ncbi.nlm.nih.gov/pubmed/33659715 http://dx.doi.org/10.1016/j.bbrep.2021.100952 |
_version_ | 1783652503523426304 |
---|---|
author | Yang, Ning Zhang, Yue Chen, Lu Wang, Wei Liu, Ruirui Gao, Run Zhou, Yaping Li, Hui |
author_facet | Yang, Ning Zhang, Yue Chen, Lu Wang, Wei Liu, Ruirui Gao, Run Zhou, Yaping Li, Hui |
author_sort | Yang, Ning |
collection | PubMed |
description | Jasmonic acid (JA) is regarded as an endogenous regulator which plays an important role in regulating plant growth, development and stress response. Using the seedlings of A. thaliana ecotype Col-0 (wild-type, WT), phospholipase Dδ (PLDδ) deficient mutant (pldδ), the G protein α subunit (GPA1) deficient mutant (gpa1-4), 9-Lipoxygenase (9-LOX) deficient mutants (lox1 and lox5) as materials, the effects of JA responding to osmotic stress and the functions of G protein and PLDδ in this response were investigated. The results showed that GPA1 involved in the regulation of JA to PLDδ under osmotic stress. Both GPA1 and PLDδ participated in the regulation of JA on the seed germination and osmotic tolerance. Exogenous MeJA reduced the EL and MDA in WT, but increased the EL and MDA in gpa1-4 and pldδ, indicating that GPA1 and PLDδ were involved in the protection of JA on the membrane. The genes expression levels, and the activities of PLDδ and LOX1 were significantly induced by osmotic stress. The LOX activity and JA content in pldδ seedings were lower obviously than those in WT, but were markedly increased and were higher than WT after applying phosphatidic acid (PA). These results demonstrated that JA responded to osmotic stress by regulating G protein and PLDδ in A. thaliana. PLDδ was located upstream of 9-LOX and involved in the JA biosynthesis. |
format | Online Article Text |
id | pubmed-7890375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-78903752021-03-02 G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana Yang, Ning Zhang, Yue Chen, Lu Wang, Wei Liu, Ruirui Gao, Run Zhou, Yaping Li, Hui Biochem Biophys Rep Research Article Jasmonic acid (JA) is regarded as an endogenous regulator which plays an important role in regulating plant growth, development and stress response. Using the seedlings of A. thaliana ecotype Col-0 (wild-type, WT), phospholipase Dδ (PLDδ) deficient mutant (pldδ), the G protein α subunit (GPA1) deficient mutant (gpa1-4), 9-Lipoxygenase (9-LOX) deficient mutants (lox1 and lox5) as materials, the effects of JA responding to osmotic stress and the functions of G protein and PLDδ in this response were investigated. The results showed that GPA1 involved in the regulation of JA to PLDδ under osmotic stress. Both GPA1 and PLDδ participated in the regulation of JA on the seed germination and osmotic tolerance. Exogenous MeJA reduced the EL and MDA in WT, but increased the EL and MDA in gpa1-4 and pldδ, indicating that GPA1 and PLDδ were involved in the protection of JA on the membrane. The genes expression levels, and the activities of PLDδ and LOX1 were significantly induced by osmotic stress. The LOX activity and JA content in pldδ seedings were lower obviously than those in WT, but were markedly increased and were higher than WT after applying phosphatidic acid (PA). These results demonstrated that JA responded to osmotic stress by regulating G protein and PLDδ in A. thaliana. PLDδ was located upstream of 9-LOX and involved in the JA biosynthesis. Elsevier 2021-02-12 /pmc/articles/PMC7890375/ /pubmed/33659715 http://dx.doi.org/10.1016/j.bbrep.2021.100952 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Yang, Ning Zhang, Yue Chen, Lu Wang, Wei Liu, Ruirui Gao, Run Zhou, Yaping Li, Hui G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana |
title | G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana |
title_full | G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana |
title_fullStr | G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana |
title_full_unstemmed | G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana |
title_short | G protein and PLDδ are involved in JA to regulate osmotic stress responses in Arabidopsis thaliana |
title_sort | g protein and pldδ are involved in ja to regulate osmotic stress responses in arabidopsis thaliana |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890375/ https://www.ncbi.nlm.nih.gov/pubmed/33659715 http://dx.doi.org/10.1016/j.bbrep.2021.100952 |
work_keys_str_mv | AT yangning gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana AT zhangyue gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana AT chenlu gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana AT wangwei gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana AT liuruirui gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana AT gaorun gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana AT zhouyaping gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana AT lihui gproteinandplddareinvolvedinjatoregulateosmoticstressresponsesinarabidopsisthaliana |