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Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study

Methamphetamine (MA) is the largest drug threat across the globe, with health effects including neurotoxicity and cardiovascular disease. Recent studies have begun to link microRNAs (miRNAs) to the processes related to MA use and addiction. Our studies are the first to analyse plasma EVs and their m...

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Autores principales: Sandau, Ursula S., Duggan, Erika, Shi, Xiao, Smith, Sierra J., Huckans, Marilyn, Schutzer, William E., Loftis, Jennifer M., Janowsky, Aaron, Nolan, John P., Saugstad, Julie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890470/
https://www.ncbi.nlm.nih.gov/pubmed/33613872
http://dx.doi.org/10.1002/jev2.12028
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author Sandau, Ursula S.
Duggan, Erika
Shi, Xiao
Smith, Sierra J.
Huckans, Marilyn
Schutzer, William E.
Loftis, Jennifer M.
Janowsky, Aaron
Nolan, John P.
Saugstad, Julie A.
author_facet Sandau, Ursula S.
Duggan, Erika
Shi, Xiao
Smith, Sierra J.
Huckans, Marilyn
Schutzer, William E.
Loftis, Jennifer M.
Janowsky, Aaron
Nolan, John P.
Saugstad, Julie A.
author_sort Sandau, Ursula S.
collection PubMed
description Methamphetamine (MA) is the largest drug threat across the globe, with health effects including neurotoxicity and cardiovascular disease. Recent studies have begun to link microRNAs (miRNAs) to the processes related to MA use and addiction. Our studies are the first to analyse plasma EVs and their miRNA cargo in humans actively using MA (MA‐ACT) and control participants (CTL). In this cohort we also assessed the effects of tobacco use on plasma EVs. We used vesicle flow cytometry to show that the MA‐ACT group had an increased abundance of EV tetraspanin markers (CD9, CD63, CD81), but not pro‐coagulant, platelet‐, and red blood cell‐derived EVs. We also found that of the 169 plasma EV miRNAs, eight were of interest in MA‐ACT based on multiple statistical criteria. In smokers, we identified 15 miRNAs of interest, two that overlapped with the eight MA‐ACT miRNAs. Three of the MA‐ACT miRNAs significantly correlated with clinical features of MA use and target prediction with these miRNAs identified pathways implicated in MA use, including cardiovascular disease and neuroinflammation. Together our findings indicate that MA use regulates EVs and their miRNA cargo, and support that further studies are warranted to investigate their mechanistic role in addiction, recovery, and recidivism.
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spelling pubmed-78904702021-02-18 Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study Sandau, Ursula S. Duggan, Erika Shi, Xiao Smith, Sierra J. Huckans, Marilyn Schutzer, William E. Loftis, Jennifer M. Janowsky, Aaron Nolan, John P. Saugstad, Julie A. J Extracell Vesicles Research Articles Methamphetamine (MA) is the largest drug threat across the globe, with health effects including neurotoxicity and cardiovascular disease. Recent studies have begun to link microRNAs (miRNAs) to the processes related to MA use and addiction. Our studies are the first to analyse plasma EVs and their miRNA cargo in humans actively using MA (MA‐ACT) and control participants (CTL). In this cohort we also assessed the effects of tobacco use on plasma EVs. We used vesicle flow cytometry to show that the MA‐ACT group had an increased abundance of EV tetraspanin markers (CD9, CD63, CD81), but not pro‐coagulant, platelet‐, and red blood cell‐derived EVs. We also found that of the 169 plasma EV miRNAs, eight were of interest in MA‐ACT based on multiple statistical criteria. In smokers, we identified 15 miRNAs of interest, two that overlapped with the eight MA‐ACT miRNAs. Three of the MA‐ACT miRNAs significantly correlated with clinical features of MA use and target prediction with these miRNAs identified pathways implicated in MA use, including cardiovascular disease and neuroinflammation. Together our findings indicate that MA use regulates EVs and their miRNA cargo, and support that further studies are warranted to investigate their mechanistic role in addiction, recovery, and recidivism. John Wiley and Sons Inc. 2020-11-28 2020-11 /pmc/articles/PMC7890470/ /pubmed/33613872 http://dx.doi.org/10.1002/jev2.12028 Text en © 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Sandau, Ursula S.
Duggan, Erika
Shi, Xiao
Smith, Sierra J.
Huckans, Marilyn
Schutzer, William E.
Loftis, Jennifer M.
Janowsky, Aaron
Nolan, John P.
Saugstad, Julie A.
Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study
title Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study
title_full Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study
title_fullStr Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study
title_full_unstemmed Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study
title_short Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study
title_sort methamphetamine use alters human plasma extracellular vesicles and their microrna cargo: an exploratory study
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890470/
https://www.ncbi.nlm.nih.gov/pubmed/33613872
http://dx.doi.org/10.1002/jev2.12028
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