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Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer
Branched poly(N-isopropylacrylamide) was functionalized with Amphotericin B (AmB) at the chain ends to produce an antifungal material. The polymer showed antifungal properties against AmB-sensitive strains of Candida albicans, Fusarium keratoplasticum and Aspergillus flavus (minimal inhibitory conce...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890487/ https://www.ncbi.nlm.nih.gov/pubmed/33614095 http://dx.doi.org/10.1098/rsos.201655 |
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author | Swift, Thomas Caseley, Emily Pinnock, Abbigail Shepherd, Joanna Shivshetty, Nagaveni Garg, Prashant Ian Douglas, C. W. MacNeil, Sheila Rimmer, Stephen |
author_facet | Swift, Thomas Caseley, Emily Pinnock, Abbigail Shepherd, Joanna Shivshetty, Nagaveni Garg, Prashant Ian Douglas, C. W. MacNeil, Sheila Rimmer, Stephen |
author_sort | Swift, Thomas |
collection | PubMed |
description | Branched poly(N-isopropylacrylamide) was functionalized with Amphotericin B (AmB) at the chain ends to produce an antifungal material. The polymer showed antifungal properties against AmB-sensitive strains of Candida albicans, Fusarium keratoplasticum and Aspergillus flavus (minimal inhibitory concentration ranged from 5 to 500 µg ml(−1)) but was not effective against an AmB resistant strain of C. albicans nor against Candida tropicalis. The polymer end groups bound to the AmB target, ergosterol, and the fluorescence spectrum of a dye used as a solvatochromic probe, Nile red, was blue shifted indicating that segments of the polymer became desolvated on binding. The polymer was less toxic to corneal and renal epithelial cells and explanted corneal tissue than the free drug. Also, the polymer did not induce reactive oxygen species release from peripheral blood mononuclear cells, nor did it cause a substantial release of the proinflammatory cytokines, tumour necrosis factor-α and interleukin-1β (at 0.5 mg ml(−1)). |
format | Online Article Text |
id | pubmed-7890487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-78904872021-02-18 Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer Swift, Thomas Caseley, Emily Pinnock, Abbigail Shepherd, Joanna Shivshetty, Nagaveni Garg, Prashant Ian Douglas, C. W. MacNeil, Sheila Rimmer, Stephen R Soc Open Sci Chemistry Branched poly(N-isopropylacrylamide) was functionalized with Amphotericin B (AmB) at the chain ends to produce an antifungal material. The polymer showed antifungal properties against AmB-sensitive strains of Candida albicans, Fusarium keratoplasticum and Aspergillus flavus (minimal inhibitory concentration ranged from 5 to 500 µg ml(−1)) but was not effective against an AmB resistant strain of C. albicans nor against Candida tropicalis. The polymer end groups bound to the AmB target, ergosterol, and the fluorescence spectrum of a dye used as a solvatochromic probe, Nile red, was blue shifted indicating that segments of the polymer became desolvated on binding. The polymer was less toxic to corneal and renal epithelial cells and explanted corneal tissue than the free drug. Also, the polymer did not induce reactive oxygen species release from peripheral blood mononuclear cells, nor did it cause a substantial release of the proinflammatory cytokines, tumour necrosis factor-α and interleukin-1β (at 0.5 mg ml(−1)). The Royal Society 2021-01-13 /pmc/articles/PMC7890487/ /pubmed/33614095 http://dx.doi.org/10.1098/rsos.201655 Text en © 2021 The Authors. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Chemistry Swift, Thomas Caseley, Emily Pinnock, Abbigail Shepherd, Joanna Shivshetty, Nagaveni Garg, Prashant Ian Douglas, C. W. MacNeil, Sheila Rimmer, Stephen Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer |
title | Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer |
title_full | Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer |
title_fullStr | Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer |
title_full_unstemmed | Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer |
title_short | Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer |
title_sort | branched amphotericin functional poly(n-isopropyl acrylamide): an antifungal polymer |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890487/ https://www.ncbi.nlm.nih.gov/pubmed/33614095 http://dx.doi.org/10.1098/rsos.201655 |
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