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Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity
Glycyl‐tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diamet...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890555/ https://www.ncbi.nlm.nih.gov/pubmed/33708357 http://dx.doi.org/10.1002/jev2.12029 |
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author | Goughnour, Peter C. Park, Min Chul Kim, Sang Bum Jun, Sangmi Yang, Won Suk Chae, Sehyun Cho, Seongmin Song, Chihong Lee, Ji‐Hyun Hyun, Jae Kyung Kim, Byung Gyu Hwang, Daehee Jung, Hyun Suk Gho, Yong Song Kim, Sunghoon |
author_facet | Goughnour, Peter C. Park, Min Chul Kim, Sang Bum Jun, Sangmi Yang, Won Suk Chae, Sehyun Cho, Seongmin Song, Chihong Lee, Ji‐Hyun Hyun, Jae Kyung Kim, Byung Gyu Hwang, Daehee Jung, Hyun Suk Gho, Yong Song Kim, Sunghoon |
author_sort | Goughnour, Peter C. |
collection | PubMed |
description | Glycyl‐tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13–1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1‐containing EVs (GARS1‐EVs). Among the identified factors, insulin‐like growth factor II receptor, and vimentin also contributed to the anti‐cancer activity of GARS1‐EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis. |
format | Online Article Text |
id | pubmed-7890555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78905552021-03-10 Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity Goughnour, Peter C. Park, Min Chul Kim, Sang Bum Jun, Sangmi Yang, Won Suk Chae, Sehyun Cho, Seongmin Song, Chihong Lee, Ji‐Hyun Hyun, Jae Kyung Kim, Byung Gyu Hwang, Daehee Jung, Hyun Suk Gho, Yong Song Kim, Sunghoon J Extracell Vesicles Research Articles Glycyl‐tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13–1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1‐containing EVs (GARS1‐EVs). Among the identified factors, insulin‐like growth factor II receptor, and vimentin also contributed to the anti‐cancer activity of GARS1‐EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis. John Wiley and Sons Inc. 2020-12-01 2020-11 /pmc/articles/PMC7890555/ /pubmed/33708357 http://dx.doi.org/10.1002/jev2.12029 Text en © 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Research Articles Goughnour, Peter C. Park, Min Chul Kim, Sang Bum Jun, Sangmi Yang, Won Suk Chae, Sehyun Cho, Seongmin Song, Chihong Lee, Ji‐Hyun Hyun, Jae Kyung Kim, Byung Gyu Hwang, Daehee Jung, Hyun Suk Gho, Yong Song Kim, Sunghoon Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity |
title | Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity |
title_full | Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity |
title_fullStr | Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity |
title_full_unstemmed | Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity |
title_short | Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity |
title_sort | extracellular vesicles derived from macrophages display glycyl‐trna synthetase 1 and exhibit anti‐cancer activity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890555/ https://www.ncbi.nlm.nih.gov/pubmed/33708357 http://dx.doi.org/10.1002/jev2.12029 |
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