Cargando…

Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis

BACKGROUND: Microbial keratitis is a leading cause of preventable blindness worldwide. Conventional sampling and culture techniques are time-consuming, with over 40% of cases being culture-negative. Nanopore sequencing technology is portable and capable of generating long sequencing reads in real-ti...

Descripción completa

Detalles Bibliográficos
Autores principales:	Low, Liying, Fuentes-Utrilla, Pablo, Hodson, James, O’Neil, John D., Rossiter, Amanda E., Begum, Ghazala, Suleiman, Kusy, Murray, Philip I., Wallace, Graham R., Loman, Nicholas J., Rauz, Saaeha
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	PeerJ Inc. 2021
Materias:	Bioinformatics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891086/ https://www.ncbi.nlm.nih.gov/pubmed/33628638 http://dx.doi.org/10.7717/peerj.10778

Ejemplares similares

Gut Dysbiosis in Ocular Mucous Membrane Pemphigoid
por: Low, Liying, et al.
Publicado: (2022)

The impact of the COVID-19 pandemic on microbial keratitis presentation patterns
por: Butt, Gibran F., et al.
Publicado: (2021)

Calculating the economic burden of presumed microbial keratitis admissions at a tertiary referral centre in the UK
por: Moussa, George, et al.
Publicado: (2020)

Comparative full-length transcriptome analysis by Oxford Nanopore Technologies reveals genes involved in anthocyanin accumulation in storage roots of sweet potatoes (Ipomoea batatas L.)
por: Xiong, Jun, et al.
Publicado: (2022)

16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis
por: Zhang, Ke, et al.
Publicado: (2022)

Full-length transcriptome analysis of Adiantum flabellulatum gametophyte
por: Cai, Zeping, et al.
Publicado: (2022)

tailfindr: alignment-free poly(A) length measurement for Oxford Nanopore RNA and DNA sequencing
por: Krause, Maximilian, et al.
Publicado: (2019)

Deep metagenomic sequencing for endophthalmitis pathogen detection using a nanopore platform
por: Low, Liying, et al.
Publicado: (2022)

A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq
por: Burke, Catherine M., et al.
Publicado: (2016)

Kelpie: generating full-length ‘amplicons’ from whole-metagenome datasets
por: Greenfield, Paul, et al.
Publicado: (2019)

De novo species identification using 16S rRNA gene nanopore sequencing
por: Angell, Inga Leena, et al.
Publicado: (2020)

Low density neutrophils are increased in patients with Behçet’s disease but do not explain differences in neutrophil function
por: Murad, Mariam, et al.
Publicado: (2022)

SMRT sequencing of full-length transcriptome and gene expression analysis in two chemical types of Pogostemon cablin (Blanco) Benth.
por: Zhang, Hongyi, et al.
Publicado: (2022)

Full-length genome characterization and phylogenetic analysis of SARS-CoV-2 virus strains from Yogyakarta and Central Java, Indonesia
por: Gunadi,, et al.
Publicado: (2020)

Public perceptions of eye symptoms and hospital services during the first UK lockdown of the COVID-19 pandemic: a web survey study
por: Butt, Gibran F, et al.
Publicado: (2021)

Single-cell transcriptome sequencing on the Nanopore platform with ScNapBar
por: Wang, Qi, et al.
Publicado: (2021)

Barcode demultiplexing of nanopore sequencing raw signals by unsupervised machine learning
por: Papetti, Daniele M., et al.
Publicado: (2023)

Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
por: Hargreaves, Adam D., et al.
Publicado: (2015)

Anxiety and depression in inflammatory eye disease: exploring the potential impact of topical treatment frequency as a putative psychometric item
por: Vakros, Georgios, et al.
Publicado: (2021)

Rapid assessment of ocular drug delivery in a novel ex vivo corneal model
por: Begum, Ghazala, et al.
Publicado: (2020)

NanoForms: an integrated server for processing, analysis and assembly of raw sequencing data of microbial genomes, from Oxford Nanopore technology
por: Czmil, Anna, et al.
Publicado: (2022)

FUCHS—towards full circular RNA characterization using RNAseq
por: Metge, Franziska, et al.
Publicado: (2017)

Identifying effects of genetic obesity exposure on leukocyte telomere length using Mendelian randomization
por: Wan, Bangbei, et al.
Publicado: (2023)

Full genome sequence analysis of a 1-7-4-like PRRSV strain in Fujian Province, China
por: Liu, Jiankui, et al.
Publicado: (2019)

Time-calibrated phylogeny and full mitogenome sequence of the Galapagos sea lion (Zalophus wollebaeki) from scat DNA
por: Asadobay, Pacarina, et al.
Publicado: (2023)

Lid opening and conformational stability of T1 Lipase is mediated by increasing chain length polar solvents
por: Maiangwa, Jonathan, et al.
Publicado: (2017)

stepRNA: Identification of Dicer cleavage signatures and passenger strand lengths in small RNA sequences
por: Murcott, Ben, et al.
Publicado: (2022)

Prediction uncertainty assessment of a systems biology model requires a sample of the full probability distribution of its parameters
por: van Mourik, Simon, et al.
Publicado: (2014)

Gene length is a pivotal feature to explain disparities in transcript capture between single transcriptome techniques
por: Pavan, Ricardo R., et al.
Publicado: (2023)

An interpretable time series machine learning method for varying forecast and nowcast lengths in wastewater-based epidemiology
por: Lai, Mallory, et al.
Publicado: (2023)

Computational inference of H3K4me3 and H3K27ac domain length
por: Zubek, Julian, et al.
Publicado: (2016)

Improved Nanopore full-length cDNA sequencing by PCR-suppression
por: Bayega, Anthony, et al.
Publicado: (2022)

Direct Nanopore Sequencing of Individual Full Length tRNA Strands
por: Thomas, Niki K., et al.
Publicado: (2021)

Comment on: “Controversies regarding mask usage in ophthalmic units in the United Kingdom during the COVID-19 pandemic”
por: Panthagani, Jesse, et al.
Publicado: (2020)

Integrative analysis of common genes and driver mutations implicated in hormone stimulation for four cancers in women
por: Bhyan, Salma Begum, et al.
Publicado: (2019)

Genetic links between endometriosis and cancers in women
por: Bhyan, Salma Begum, et al.
Publicado: (2019)

GFVO: the Genomic Feature and Variation Ontology
por: Baran, Joachim, et al.
Publicado: (2015)

Longer study length, standardized sampling techniques, and broader geographic scope leads to higher likelihood of detecting stable abundance patterns in long term black-legged tick studies
por: Christie, Rowan, et al.
Publicado: (2022)

16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences
por: Hsieh, Yu-Peng, et al.
Publicado: (2022)

Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance in Arabidopsis thaliana
por: Jiang, Shuna, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_fd9hp7sonfs9jr07cvfeormns0