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Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep

Insulin-like growth factor 1 (IGF1) is a mediator of the effects of growth hormone and polymorphism in the IGF1 gene (IGF1) is reported to affect fat deposition in some livestock species. In this study, nucleotide sequence variation in three regions of ovine IGF1 (part of the 5′ flanking region, the...

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Autores principales: Li, Shaobin, Zhou, Huitong, Zhao, Fangfang, Fang, Qian, Wang, Jiqing, Liu, Xiu, Luo, Yuzhu, Hickford, Jon G.H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891192/
https://www.ncbi.nlm.nih.gov/pubmed/33325787
http://dx.doi.org/10.1089/dna.2020.6166
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author Li, Shaobin
Zhou, Huitong
Zhao, Fangfang
Fang, Qian
Wang, Jiqing
Liu, Xiu
Luo, Yuzhu
Hickford, Jon G.H.
author_facet Li, Shaobin
Zhou, Huitong
Zhao, Fangfang
Fang, Qian
Wang, Jiqing
Liu, Xiu
Luo, Yuzhu
Hickford, Jon G.H.
author_sort Li, Shaobin
collection PubMed
description Insulin-like growth factor 1 (IGF1) is a mediator of the effects of growth hormone and polymorphism in the IGF1 gene (IGF1) is reported to affect fat deposition in some livestock species. In this study, nucleotide sequence variation in three regions of ovine IGF1 (part of the 5′ flanking region, the exon 3 region, and the exon 4 region) was investigated in 848 New Zealand Romney lambs using PCR-single strand conformation polymorphism (SSCP) analyses to ascertain if single nucleotide polymorphisms (SNPs) existed. Six SNPs were identified across these three regions. The effect of the sequence variation in the exon 3 and exon 4 regions on growth and carcass traits were investigated. One of the PCR-SSCP sequence variants in the exon 3 region was associated with variation in hot carcass weight, carcass fat depth at the 12th rib measured using video imaging and the percentage proportion of leg lean meat, whereas the other was associated with variation in growth rate to weaning. No associations were detected for the other gene regions analyzed. The results suggest that polymorphism in exon 3 of ovine IGF1 has potential for use as a gene-marker for some carcass and growth traits.
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spelling pubmed-78911922021-02-19 Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep Li, Shaobin Zhou, Huitong Zhao, Fangfang Fang, Qian Wang, Jiqing Liu, Xiu Luo, Yuzhu Hickford, Jon G.H. DNA Cell Biol Molecular Genetics/Genomics/Epigenetics Insulin-like growth factor 1 (IGF1) is a mediator of the effects of growth hormone and polymorphism in the IGF1 gene (IGF1) is reported to affect fat deposition in some livestock species. In this study, nucleotide sequence variation in three regions of ovine IGF1 (part of the 5′ flanking region, the exon 3 region, and the exon 4 region) was investigated in 848 New Zealand Romney lambs using PCR-single strand conformation polymorphism (SSCP) analyses to ascertain if single nucleotide polymorphisms (SNPs) existed. Six SNPs were identified across these three regions. The effect of the sequence variation in the exon 3 and exon 4 regions on growth and carcass traits were investigated. One of the PCR-SSCP sequence variants in the exon 3 region was associated with variation in hot carcass weight, carcass fat depth at the 12th rib measured using video imaging and the percentage proportion of leg lean meat, whereas the other was associated with variation in growth rate to weaning. No associations were detected for the other gene regions analyzed. The results suggest that polymorphism in exon 3 of ovine IGF1 has potential for use as a gene-marker for some carcass and growth traits. Mary Ann Liebert, Inc., publishers 2021-02-01 2021-02-11 /pmc/articles/PMC7891192/ /pubmed/33325787 http://dx.doi.org/10.1089/dna.2020.6166 Text en © Shaobin Li et al. 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Genetics/Genomics/Epigenetics
Li, Shaobin
Zhou, Huitong
Zhao, Fangfang
Fang, Qian
Wang, Jiqing
Liu, Xiu
Luo, Yuzhu
Hickford, Jon G.H.
Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep
title Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep
title_full Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep
title_fullStr Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep
title_full_unstemmed Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep
title_short Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep
title_sort nucleotide sequence variation in the insulin-like growth factor 1 gene affects growth and carcass traits in new zealand romney sheep
topic Molecular Genetics/Genomics/Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891192/
https://www.ncbi.nlm.nih.gov/pubmed/33325787
http://dx.doi.org/10.1089/dna.2020.6166
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