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Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep
Insulin-like growth factor 1 (IGF1) is a mediator of the effects of growth hormone and polymorphism in the IGF1 gene (IGF1) is reported to affect fat deposition in some livestock species. In this study, nucleotide sequence variation in three regions of ovine IGF1 (part of the 5′ flanking region, the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mary Ann Liebert, Inc., publishers
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891192/ https://www.ncbi.nlm.nih.gov/pubmed/33325787 http://dx.doi.org/10.1089/dna.2020.6166 |
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author | Li, Shaobin Zhou, Huitong Zhao, Fangfang Fang, Qian Wang, Jiqing Liu, Xiu Luo, Yuzhu Hickford, Jon G.H. |
author_facet | Li, Shaobin Zhou, Huitong Zhao, Fangfang Fang, Qian Wang, Jiqing Liu, Xiu Luo, Yuzhu Hickford, Jon G.H. |
author_sort | Li, Shaobin |
collection | PubMed |
description | Insulin-like growth factor 1 (IGF1) is a mediator of the effects of growth hormone and polymorphism in the IGF1 gene (IGF1) is reported to affect fat deposition in some livestock species. In this study, nucleotide sequence variation in three regions of ovine IGF1 (part of the 5′ flanking region, the exon 3 region, and the exon 4 region) was investigated in 848 New Zealand Romney lambs using PCR-single strand conformation polymorphism (SSCP) analyses to ascertain if single nucleotide polymorphisms (SNPs) existed. Six SNPs were identified across these three regions. The effect of the sequence variation in the exon 3 and exon 4 regions on growth and carcass traits were investigated. One of the PCR-SSCP sequence variants in the exon 3 region was associated with variation in hot carcass weight, carcass fat depth at the 12th rib measured using video imaging and the percentage proportion of leg lean meat, whereas the other was associated with variation in growth rate to weaning. No associations were detected for the other gene regions analyzed. The results suggest that polymorphism in exon 3 of ovine IGF1 has potential for use as a gene-marker for some carcass and growth traits. |
format | Online Article Text |
id | pubmed-7891192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Mary Ann Liebert, Inc., publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-78911922021-02-19 Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep Li, Shaobin Zhou, Huitong Zhao, Fangfang Fang, Qian Wang, Jiqing Liu, Xiu Luo, Yuzhu Hickford, Jon G.H. DNA Cell Biol Molecular Genetics/Genomics/Epigenetics Insulin-like growth factor 1 (IGF1) is a mediator of the effects of growth hormone and polymorphism in the IGF1 gene (IGF1) is reported to affect fat deposition in some livestock species. In this study, nucleotide sequence variation in three regions of ovine IGF1 (part of the 5′ flanking region, the exon 3 region, and the exon 4 region) was investigated in 848 New Zealand Romney lambs using PCR-single strand conformation polymorphism (SSCP) analyses to ascertain if single nucleotide polymorphisms (SNPs) existed. Six SNPs were identified across these three regions. The effect of the sequence variation in the exon 3 and exon 4 regions on growth and carcass traits were investigated. One of the PCR-SSCP sequence variants in the exon 3 region was associated with variation in hot carcass weight, carcass fat depth at the 12th rib measured using video imaging and the percentage proportion of leg lean meat, whereas the other was associated with variation in growth rate to weaning. No associations were detected for the other gene regions analyzed. The results suggest that polymorphism in exon 3 of ovine IGF1 has potential for use as a gene-marker for some carcass and growth traits. Mary Ann Liebert, Inc., publishers 2021-02-01 2021-02-11 /pmc/articles/PMC7891192/ /pubmed/33325787 http://dx.doi.org/10.1089/dna.2020.6166 Text en © Shaobin Li et al. 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Genetics/Genomics/Epigenetics Li, Shaobin Zhou, Huitong Zhao, Fangfang Fang, Qian Wang, Jiqing Liu, Xiu Luo, Yuzhu Hickford, Jon G.H. Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep |
title | Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep |
title_full | Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep |
title_fullStr | Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep |
title_full_unstemmed | Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep |
title_short | Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep |
title_sort | nucleotide sequence variation in the insulin-like growth factor 1 gene affects growth and carcass traits in new zealand romney sheep |
topic | Molecular Genetics/Genomics/Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891192/ https://www.ncbi.nlm.nih.gov/pubmed/33325787 http://dx.doi.org/10.1089/dna.2020.6166 |
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