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Cryo‐FIB preparation of whole cells and tissue for cryo‐TEM: use of high‐pressure frozen specimens in tubes and planchets

The desire to study macromolecular complexes within their cellular context requires the ability to produce thin samples suitable for cryo‐TEM (cryo‐transmission electron microscope) investigations. In this paper, we discuss two similar approaches, which were developed independently in Utrecht (the N...

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Detalles Bibliográficos
Autores principales: DE WINTER, D.A.M., HSIEH, C., MARKO, M., HAYLES, M. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891314/
https://www.ncbi.nlm.nih.gov/pubmed/32691851
http://dx.doi.org/10.1111/jmi.12943

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