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Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity

Exposure of human immune cells to asbestos causes a reduction in antitumor immunity. The present study aimed to investigate the recovery of reduced antitumor immunity by several ingredients taken as supplements or foods, including trehalose (Treh) and glycosylated hesperidin (gHesp). Peripheral bloo...

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Autores principales: Yamamoto, Shoko, Lee, Suni, Ariyasu, Toshio, Endo, Shin, Miyata, Satomi, Yasuda, Akiko, Harashima, Akira, Ohta, Tsunetaka, Kumagai-Takei, Naoko, Ito, Tatsuo, Shimizu, Yurika, Srinivas, Bandaru, Sada, Nagisa, Nishimura, Yasumitsu, Otsuki, Takemi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891817/
https://www.ncbi.nlm.nih.gov/pubmed/33655329
http://dx.doi.org/10.3892/ijo.2021.5182
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author Yamamoto, Shoko
Lee, Suni
Ariyasu, Toshio
Endo, Shin
Miyata, Satomi
Yasuda, Akiko
Harashima, Akira
Ohta, Tsunetaka
Kumagai-Takei, Naoko
Ito, Tatsuo
Shimizu, Yurika
Srinivas, Bandaru
Sada, Nagisa
Nishimura, Yasumitsu
Otsuki, Takemi
author_facet Yamamoto, Shoko
Lee, Suni
Ariyasu, Toshio
Endo, Shin
Miyata, Satomi
Yasuda, Akiko
Harashima, Akira
Ohta, Tsunetaka
Kumagai-Takei, Naoko
Ito, Tatsuo
Shimizu, Yurika
Srinivas, Bandaru
Sada, Nagisa
Nishimura, Yasumitsu
Otsuki, Takemi
author_sort Yamamoto, Shoko
collection PubMed
description Exposure of human immune cells to asbestos causes a reduction in antitumor immunity. The present study aimed to investigate the recovery of reduced antitumor immunity by several ingredients taken as supplements or foods, including trehalose (Treh) and glycosylated hesperidin (gHesp). Peripheral blood CD4(+) cells were stimulated with IL-2, anti-CD3 and anti-CD28 antibodies for 3 days, followed by further stimulation with IL-2 for 7 days. Subsequently, cells were stimulated with IL-2 for an additional 28 days. During the 28 days, cells were cultured in the absence or presence of 50 μg/ml chrysotile asbestos fibers. In addition, cells were treated with 10 mM Treh or 10 μM gHesp. Following culture for 28 days, reverse transcription-quantitative PCR was performed to assess the expression levels of transcription factors, cytokines and specific genes, including matrix metalloproteinase-7 (MMP-7), nicotinamide nucleotide transhydrogenase (NNT) and C-X-C motif chemokine receptor 3, in unstimulated cells (fresh) and cells stimulated with PMA and ionomycin (stimuli). The results demonstrated that compared with the control group, chrysotile-exposure induced alterations in MMP-7, NNT and IL-17A expression levels were not observed in the 'Treh' and 'gHesp' groups in stimulated cells. The results suggested that Treh and gHesp may reverse asbestos exposure-induced reduced antitumor immunity in T helper cells. However, further investigation is required to confirm the efficacy of future trials involving the use of these compounds with high-risk human populations exposed to asbestos, such as workers involved in asbestos-handling activities.
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spelling pubmed-78918172021-03-08 Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity Yamamoto, Shoko Lee, Suni Ariyasu, Toshio Endo, Shin Miyata, Satomi Yasuda, Akiko Harashima, Akira Ohta, Tsunetaka Kumagai-Takei, Naoko Ito, Tatsuo Shimizu, Yurika Srinivas, Bandaru Sada, Nagisa Nishimura, Yasumitsu Otsuki, Takemi Int J Oncol Articles Exposure of human immune cells to asbestos causes a reduction in antitumor immunity. The present study aimed to investigate the recovery of reduced antitumor immunity by several ingredients taken as supplements or foods, including trehalose (Treh) and glycosylated hesperidin (gHesp). Peripheral blood CD4(+) cells were stimulated with IL-2, anti-CD3 and anti-CD28 antibodies for 3 days, followed by further stimulation with IL-2 for 7 days. Subsequently, cells were stimulated with IL-2 for an additional 28 days. During the 28 days, cells were cultured in the absence or presence of 50 μg/ml chrysotile asbestos fibers. In addition, cells were treated with 10 mM Treh or 10 μM gHesp. Following culture for 28 days, reverse transcription-quantitative PCR was performed to assess the expression levels of transcription factors, cytokines and specific genes, including matrix metalloproteinase-7 (MMP-7), nicotinamide nucleotide transhydrogenase (NNT) and C-X-C motif chemokine receptor 3, in unstimulated cells (fresh) and cells stimulated with PMA and ionomycin (stimuli). The results demonstrated that compared with the control group, chrysotile-exposure induced alterations in MMP-7, NNT and IL-17A expression levels were not observed in the 'Treh' and 'gHesp' groups in stimulated cells. The results suggested that Treh and gHesp may reverse asbestos exposure-induced reduced antitumor immunity in T helper cells. However, further investigation is required to confirm the efficacy of future trials involving the use of these compounds with high-risk human populations exposed to asbestos, such as workers involved in asbestos-handling activities. D.A. Spandidos 2021-02-02 /pmc/articles/PMC7891817/ /pubmed/33655329 http://dx.doi.org/10.3892/ijo.2021.5182 Text en Copyright: © Yamamoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yamamoto, Shoko
Lee, Suni
Ariyasu, Toshio
Endo, Shin
Miyata, Satomi
Yasuda, Akiko
Harashima, Akira
Ohta, Tsunetaka
Kumagai-Takei, Naoko
Ito, Tatsuo
Shimizu, Yurika
Srinivas, Bandaru
Sada, Nagisa
Nishimura, Yasumitsu
Otsuki, Takemi
Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity
title Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity
title_full Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity
title_fullStr Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity
title_full_unstemmed Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity
title_short Ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human T cells, reducing asbestos exposure-induced antitumor immunity
title_sort ingredients such as trehalose and hesperidin taken as supplements or foods reverse alterations in human t cells, reducing asbestos exposure-induced antitumor immunity
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891817/
https://www.ncbi.nlm.nih.gov/pubmed/33655329
http://dx.doi.org/10.3892/ijo.2021.5182
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