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Human primed ILCPs support endothelial activation through NF-κB signaling

Innate lymphoid cells (ILCs) represent the most recently identified subset of effector lymphocytes, with key roles in the orchestration of early immune responses. Despite their established involvement in the pathogenesis of many inflammatory disorders, the role of ILCs in cancer remains poorly defin...

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Autores principales: Vanoni, Giulia, Ercolano, Giuseppe, Candiani, Simona, Rutigliani, Mariangela, Lanata, Mariangela, Derré, Laurent, Marcenaro, Emanuela, Schneider, Pascal, Romero, Pedro, Jandus, Camilla, Trabanelli, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891932/
https://www.ncbi.nlm.nih.gov/pubmed/33554861
http://dx.doi.org/10.7554/eLife.58838
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author Vanoni, Giulia
Ercolano, Giuseppe
Candiani, Simona
Rutigliani, Mariangela
Lanata, Mariangela
Derré, Laurent
Marcenaro, Emanuela
Schneider, Pascal
Romero, Pedro
Jandus, Camilla
Trabanelli, Sara
author_facet Vanoni, Giulia
Ercolano, Giuseppe
Candiani, Simona
Rutigliani, Mariangela
Lanata, Mariangela
Derré, Laurent
Marcenaro, Emanuela
Schneider, Pascal
Romero, Pedro
Jandus, Camilla
Trabanelli, Sara
author_sort Vanoni, Giulia
collection PubMed
description Innate lymphoid cells (ILCs) represent the most recently identified subset of effector lymphocytes, with key roles in the orchestration of early immune responses. Despite their established involvement in the pathogenesis of many inflammatory disorders, the role of ILCs in cancer remains poorly defined. Here we assessed whether human ILCs can actively interact with the endothelium to promote tumor growth control, favoring immune cell adhesion. We show that, among all ILC subsets, ILCPs elicited the strongest upregulation of adhesion molecules in endothelial cells (ECs) in vitro, mainly in a contact-dependent manner through the tumor necrosis factor receptor- and RANK-dependent engagement of the NF-κB pathway. Moreover, the ILCP-mediated activation of the ECs resulted to be functional by fostering the adhesion of other innate and adaptive immune cells. Interestingly, pre-exposure of ILCPs to human tumor cell lines strongly impaired this capacity. Hence, the ILCP–EC interaction might represent an attractive target to regulate the immune cell trafficking to tumor sites and, therefore, the establishment of an anti-tumor immune response.
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spelling pubmed-78919322021-02-22 Human primed ILCPs support endothelial activation through NF-κB signaling Vanoni, Giulia Ercolano, Giuseppe Candiani, Simona Rutigliani, Mariangela Lanata, Mariangela Derré, Laurent Marcenaro, Emanuela Schneider, Pascal Romero, Pedro Jandus, Camilla Trabanelli, Sara eLife Cancer Biology Innate lymphoid cells (ILCs) represent the most recently identified subset of effector lymphocytes, with key roles in the orchestration of early immune responses. Despite their established involvement in the pathogenesis of many inflammatory disorders, the role of ILCs in cancer remains poorly defined. Here we assessed whether human ILCs can actively interact with the endothelium to promote tumor growth control, favoring immune cell adhesion. We show that, among all ILC subsets, ILCPs elicited the strongest upregulation of adhesion molecules in endothelial cells (ECs) in vitro, mainly in a contact-dependent manner through the tumor necrosis factor receptor- and RANK-dependent engagement of the NF-κB pathway. Moreover, the ILCP-mediated activation of the ECs resulted to be functional by fostering the adhesion of other innate and adaptive immune cells. Interestingly, pre-exposure of ILCPs to human tumor cell lines strongly impaired this capacity. Hence, the ILCP–EC interaction might represent an attractive target to regulate the immune cell trafficking to tumor sites and, therefore, the establishment of an anti-tumor immune response. eLife Sciences Publications, Ltd 2021-02-08 /pmc/articles/PMC7891932/ /pubmed/33554861 http://dx.doi.org/10.7554/eLife.58838 Text en © 2021, Vanoni et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cancer Biology
Vanoni, Giulia
Ercolano, Giuseppe
Candiani, Simona
Rutigliani, Mariangela
Lanata, Mariangela
Derré, Laurent
Marcenaro, Emanuela
Schneider, Pascal
Romero, Pedro
Jandus, Camilla
Trabanelli, Sara
Human primed ILCPs support endothelial activation through NF-κB signaling
title Human primed ILCPs support endothelial activation through NF-κB signaling
title_full Human primed ILCPs support endothelial activation through NF-κB signaling
title_fullStr Human primed ILCPs support endothelial activation through NF-κB signaling
title_full_unstemmed Human primed ILCPs support endothelial activation through NF-κB signaling
title_short Human primed ILCPs support endothelial activation through NF-κB signaling
title_sort human primed ilcps support endothelial activation through nf-κb signaling
topic Cancer Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891932/
https://www.ncbi.nlm.nih.gov/pubmed/33554861
http://dx.doi.org/10.7554/eLife.58838
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