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Revealing the biodiversity of Chilean birds through the COI barcode approach

The mitochondrial cytochrome c oxidase subunit I (COI) gene is an effective molecular tool for the estimation of genetic variation and the identification of bird species. This molecular marker is used to differentiate among Chilean bird species by analyzing barcodes for 76 species (197 individuals),...

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Autores principales: Colihueque, Nelson, Gantz, Alberto, Parraguez, Margarita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892532/
https://www.ncbi.nlm.nih.gov/pubmed/33628081
http://dx.doi.org/10.3897/zookeys.1016.51866
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author Colihueque, Nelson
Gantz, Alberto
Parraguez, Margarita
author_facet Colihueque, Nelson
Gantz, Alberto
Parraguez, Margarita
author_sort Colihueque, Nelson
collection PubMed
description The mitochondrial cytochrome c oxidase subunit I (COI) gene is an effective molecular tool for the estimation of genetic variation and the identification of bird species. This molecular marker is used to differentiate among Chilean bird species by analyzing barcodes for 76 species (197 individuals), comprising 28 species with no previous barcode data and 48 species with sequences retrieved from the BOLD and GenBank databases. The DNA barcodes correctly identified 94.7% of the species analyzed (72 of 76 species). Mean intraspecific K2P distance was 0.3% (range 0–8.7%). Within the intraspecific divergence range, three species, Phrygilus gayi, Sephanoides sephanoides and Curaeus curaeus, showed relatively high intraspecific divergence (1.5–8.7%), possibly due to the presence of a species complex or geographic isolation of sub-populations. Mean interspecific K2P distance was 24.7% (range 1.3–43.5%). Consequently, the intraspecific K2P distance showed limited overlap with interspecific K2P distance. The mean intraspecific divergence in our study was similar to that found in temperate regions of South America (0.24%). However, it was approximately one order of magnitude lower than values reported for bird species in tropical regions of northern South America (1.8–2.13%). This result suggests that bird species from Chile show low levels of genetic structure and divergence. The small overlap between intra- and inter-specific distances implies that COI barcodes could be used as an effective tool to identify nearly all the Chilean bird species analyzed.
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spelling pubmed-78925322021-02-23 Revealing the biodiversity of Chilean birds through the COI barcode approach Colihueque, Nelson Gantz, Alberto Parraguez, Margarita Zookeys Research Article The mitochondrial cytochrome c oxidase subunit I (COI) gene is an effective molecular tool for the estimation of genetic variation and the identification of bird species. This molecular marker is used to differentiate among Chilean bird species by analyzing barcodes for 76 species (197 individuals), comprising 28 species with no previous barcode data and 48 species with sequences retrieved from the BOLD and GenBank databases. The DNA barcodes correctly identified 94.7% of the species analyzed (72 of 76 species). Mean intraspecific K2P distance was 0.3% (range 0–8.7%). Within the intraspecific divergence range, three species, Phrygilus gayi, Sephanoides sephanoides and Curaeus curaeus, showed relatively high intraspecific divergence (1.5–8.7%), possibly due to the presence of a species complex or geographic isolation of sub-populations. Mean interspecific K2P distance was 24.7% (range 1.3–43.5%). Consequently, the intraspecific K2P distance showed limited overlap with interspecific K2P distance. The mean intraspecific divergence in our study was similar to that found in temperate regions of South America (0.24%). However, it was approximately one order of magnitude lower than values reported for bird species in tropical regions of northern South America (1.8–2.13%). This result suggests that bird species from Chile show low levels of genetic structure and divergence. The small overlap between intra- and inter-specific distances implies that COI barcodes could be used as an effective tool to identify nearly all the Chilean bird species analyzed. Pensoft Publishers 2021-02-11 /pmc/articles/PMC7892532/ /pubmed/33628081 http://dx.doi.org/10.3897/zookeys.1016.51866 Text en Nelson Colihueque, Alberto Gantz, Margarita Parraguez http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Colihueque, Nelson
Gantz, Alberto
Parraguez, Margarita
Revealing the biodiversity of Chilean birds through the COI barcode approach
title Revealing the biodiversity of Chilean birds through the COI barcode approach
title_full Revealing the biodiversity of Chilean birds through the COI barcode approach
title_fullStr Revealing the biodiversity of Chilean birds through the COI barcode approach
title_full_unstemmed Revealing the biodiversity of Chilean birds through the COI barcode approach
title_short Revealing the biodiversity of Chilean birds through the COI barcode approach
title_sort revealing the biodiversity of chilean birds through the coi barcode approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892532/
https://www.ncbi.nlm.nih.gov/pubmed/33628081
http://dx.doi.org/10.3897/zookeys.1016.51866
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