The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma

The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-regulation...

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Autores principales: Maleki, Parichehr, Mowla, Seyed Javad, Taheri, Mohammad, Ghafouri-Fard, Soudeh, Raheb, Jamshid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892821/
https://www.ncbi.nlm.nih.gov/pubmed/33602983
http://dx.doi.org/10.1038/s41598-021-82892-z
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author Maleki, Parichehr
Mowla, Seyed Javad
Taheri, Mohammad
Ghafouri-Fard, Soudeh
Raheb, Jamshid
author_facet Maleki, Parichehr
Mowla, Seyed Javad
Taheri, Mohammad
Ghafouri-Fard, Soudeh
Raheb, Jamshid
author_sort Maleki, Parichehr
collection PubMed
description The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-regulation of LINK-A in A549 and Calu-3 cell lines as cellular models of non-small cell lung carcinoma (NSCLC). We used the RNA interference system to knock down LINK-A. LINK-A expression was significantly reduced by siRNA transfection in A549 and Calu-3 cell lines. LINK-A down-regulation significantly reduced cell viability, colony-forming ability and cell migration, as measured by MTT, colony formation and invasion assays. Finally, cell cycle analysis and Annexin-V/7AAD staining indicated that apoptosis was influenced by LINK-A silencing. Taken together, LINK-A can be proposed as an oncogene in NSCLC.
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spelling pubmed-78928212021-02-22 The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma Maleki, Parichehr Mowla, Seyed Javad Taheri, Mohammad Ghafouri-Fard, Soudeh Raheb, Jamshid Sci Rep Article The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-regulation of LINK-A in A549 and Calu-3 cell lines as cellular models of non-small cell lung carcinoma (NSCLC). We used the RNA interference system to knock down LINK-A. LINK-A expression was significantly reduced by siRNA transfection in A549 and Calu-3 cell lines. LINK-A down-regulation significantly reduced cell viability, colony-forming ability and cell migration, as measured by MTT, colony formation and invasion assays. Finally, cell cycle analysis and Annexin-V/7AAD staining indicated that apoptosis was influenced by LINK-A silencing. Taken together, LINK-A can be proposed as an oncogene in NSCLC. Nature Publishing Group UK 2021-02-18 /pmc/articles/PMC7892821/ /pubmed/33602983 http://dx.doi.org/10.1038/s41598-021-82892-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Maleki, Parichehr
Mowla, Seyed Javad
Taheri, Mohammad
Ghafouri-Fard, Soudeh
Raheb, Jamshid
The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_full The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_fullStr The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_full_unstemmed The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_short The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma
title_sort role of long intergenic non-coding rna for kinase activation (link-a) as an oncogene in non-small cell lung carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892821/
https://www.ncbi.nlm.nih.gov/pubmed/33602983
http://dx.doi.org/10.1038/s41598-021-82892-z
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