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Transcription activation by a sliding clamp
Transcription activation of bacteriophage T4 late genes is accomplished by a transcription activation complex containing RNA polymerase (RNAP), the promoter specificity factor gp55, the coactivator gp33, and a universal component of cellular DNA replication, the sliding clamp gp45. Although genetic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892883/ https://www.ncbi.nlm.nih.gov/pubmed/33602900 http://dx.doi.org/10.1038/s41467-021-21392-0 |
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author | Shi, Jing Wen, Aijia Jin, Sha Gao, Bo Huang, Yang Feng, Yu |
author_facet | Shi, Jing Wen, Aijia Jin, Sha Gao, Bo Huang, Yang Feng, Yu |
author_sort | Shi, Jing |
collection | PubMed |
description | Transcription activation of bacteriophage T4 late genes is accomplished by a transcription activation complex containing RNA polymerase (RNAP), the promoter specificity factor gp55, the coactivator gp33, and a universal component of cellular DNA replication, the sliding clamp gp45. Although genetic and biochemical studies have elucidated many aspects of T4 late gene transcription, no precise structure of the transcription machinery in the process is available. Here, we report the cryo-EM structures of a gp55-dependent RNAP-promoter open complex and an intact gp45-dependent transcription activation complex. The structures reveal the interactions between gp55 and the promoter DNA that mediate the recognition of T4 late promoters. In addition to the σR2 homology domain, gp55 has a helix-loop-helix motif that chaperons the template-strand single-stranded DNA of the transcription bubble. Gp33 contacts both RNAP and the upstream double-stranded DNA. Gp45 encircles the DNA and tethers RNAP to it, supporting the idea that gp45 switches the promoter search from three-dimensional diffusion mode to one-dimensional scanning mode. |
format | Online Article Text |
id | pubmed-7892883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78928832021-03-03 Transcription activation by a sliding clamp Shi, Jing Wen, Aijia Jin, Sha Gao, Bo Huang, Yang Feng, Yu Nat Commun Article Transcription activation of bacteriophage T4 late genes is accomplished by a transcription activation complex containing RNA polymerase (RNAP), the promoter specificity factor gp55, the coactivator gp33, and a universal component of cellular DNA replication, the sliding clamp gp45. Although genetic and biochemical studies have elucidated many aspects of T4 late gene transcription, no precise structure of the transcription machinery in the process is available. Here, we report the cryo-EM structures of a gp55-dependent RNAP-promoter open complex and an intact gp45-dependent transcription activation complex. The structures reveal the interactions between gp55 and the promoter DNA that mediate the recognition of T4 late promoters. In addition to the σR2 homology domain, gp55 has a helix-loop-helix motif that chaperons the template-strand single-stranded DNA of the transcription bubble. Gp33 contacts both RNAP and the upstream double-stranded DNA. Gp45 encircles the DNA and tethers RNAP to it, supporting the idea that gp45 switches the promoter search from three-dimensional diffusion mode to one-dimensional scanning mode. Nature Publishing Group UK 2021-02-18 /pmc/articles/PMC7892883/ /pubmed/33602900 http://dx.doi.org/10.1038/s41467-021-21392-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shi, Jing Wen, Aijia Jin, Sha Gao, Bo Huang, Yang Feng, Yu Transcription activation by a sliding clamp |
title | Transcription activation by a sliding clamp |
title_full | Transcription activation by a sliding clamp |
title_fullStr | Transcription activation by a sliding clamp |
title_full_unstemmed | Transcription activation by a sliding clamp |
title_short | Transcription activation by a sliding clamp |
title_sort | transcription activation by a sliding clamp |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892883/ https://www.ncbi.nlm.nih.gov/pubmed/33602900 http://dx.doi.org/10.1038/s41467-021-21392-0 |
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