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Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27

Skin aging caused by UV radiation is called photoaging is characterized by skin roughness and dryness accompanied by a significant reduction of dermal collagen. Rapamycin is a macrolide immunosuppressant which has been shown to exhibit “anti-aging” effects in cells and organisms, however, its roles...

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Autores principales: Bai, Gen-Long, Wang, Ping, Huang, Xin, Wang, Zi-Yue, Cao, Di, Liu, Chuan, Liu, Yi-Yi, Li, Ruo-Lin, Chen, Ai-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892968/
https://www.ncbi.nlm.nih.gov/pubmed/33614662
http://dx.doi.org/10.3389/fcell.2021.633331
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author Bai, Gen-Long
Wang, Ping
Huang, Xin
Wang, Zi-Yue
Cao, Di
Liu, Chuan
Liu, Yi-Yi
Li, Ruo-Lin
Chen, Ai-Jun
author_facet Bai, Gen-Long
Wang, Ping
Huang, Xin
Wang, Zi-Yue
Cao, Di
Liu, Chuan
Liu, Yi-Yi
Li, Ruo-Lin
Chen, Ai-Jun
author_sort Bai, Gen-Long
collection PubMed
description Skin aging caused by UV radiation is called photoaging is characterized by skin roughness and dryness accompanied by a significant reduction of dermal collagen. Rapamycin is a macrolide immunosuppressant which has been shown to exhibit “anti-aging” effects in cells and organisms, however, its roles in the skin photoaging remains unclear. Here, we investigate the role of rapamycin and HSP27, which we have previously identified as an inhibitor of UV-induced apoptosis and senescence in HaCat cells, in a UVA-induced photoaging model of primary human dermal fibroblasts (HDFs). Results from senescence-associated beta-galactosidase (SA-β-gal) staining revealed that rapamycin significantly reduced senescence in UVA-treated HDFs. In addition, treatment with rapamycin significantly increased cell autophagy levels, decreased the expression of p53 and phosphorylated HSP27, and reduced genotoxic and oxidative cellular stress levels in UVA-induced HDFs. Knockdown of HSP27 resulted in a significant increase of MMP-1 and MMP-3 as well as a decrease in type I collagen expression. Rapamycin mitigated these effects by activation of the classical TGF-β/Smad signaling pathway and increasing the transcriptional activity of MAPK/AP-1. Taken together, these results suggest that rapamycin may potentially serve as a preventive and therapeutic agent for UVA-induced photoaging of the skin.
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spelling pubmed-78929682021-02-20 Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27 Bai, Gen-Long Wang, Ping Huang, Xin Wang, Zi-Yue Cao, Di Liu, Chuan Liu, Yi-Yi Li, Ruo-Lin Chen, Ai-Jun Front Cell Dev Biol Cell and Developmental Biology Skin aging caused by UV radiation is called photoaging is characterized by skin roughness and dryness accompanied by a significant reduction of dermal collagen. Rapamycin is a macrolide immunosuppressant which has been shown to exhibit “anti-aging” effects in cells and organisms, however, its roles in the skin photoaging remains unclear. Here, we investigate the role of rapamycin and HSP27, which we have previously identified as an inhibitor of UV-induced apoptosis and senescence in HaCat cells, in a UVA-induced photoaging model of primary human dermal fibroblasts (HDFs). Results from senescence-associated beta-galactosidase (SA-β-gal) staining revealed that rapamycin significantly reduced senescence in UVA-treated HDFs. In addition, treatment with rapamycin significantly increased cell autophagy levels, decreased the expression of p53 and phosphorylated HSP27, and reduced genotoxic and oxidative cellular stress levels in UVA-induced HDFs. Knockdown of HSP27 resulted in a significant increase of MMP-1 and MMP-3 as well as a decrease in type I collagen expression. Rapamycin mitigated these effects by activation of the classical TGF-β/Smad signaling pathway and increasing the transcriptional activity of MAPK/AP-1. Taken together, these results suggest that rapamycin may potentially serve as a preventive and therapeutic agent for UVA-induced photoaging of the skin. Frontiers Media S.A. 2021-02-05 /pmc/articles/PMC7892968/ /pubmed/33614662 http://dx.doi.org/10.3389/fcell.2021.633331 Text en Copyright © 2021 Bai, Wang, Huang, Wang, Cao, Liu, Liu, Li and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Bai, Gen-Long
Wang, Ping
Huang, Xin
Wang, Zi-Yue
Cao, Di
Liu, Chuan
Liu, Yi-Yi
Li, Ruo-Lin
Chen, Ai-Jun
Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27
title Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27
title_full Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27
title_fullStr Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27
title_full_unstemmed Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27
title_short Rapamycin Protects Skin Fibroblasts From UVA-Induced Photoaging by Inhibition of p53 and Phosphorylated HSP27
title_sort rapamycin protects skin fibroblasts from uva-induced photoaging by inhibition of p53 and phosphorylated hsp27
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892968/
https://www.ncbi.nlm.nih.gov/pubmed/33614662
http://dx.doi.org/10.3389/fcell.2021.633331
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