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Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden

Shiga toxin-producing Escherichia coli (STEC) are important foodborne pathogens that can cause human infections ranging from asymptomatic carriage to bloody diarrhea (BD) and fatal hemolytic uremic syndrome (HUS). However, the molecular mechanism of STEC pathogenesis is not entirely known. Here, we...

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Autores principales: Bai, Xiangning, Zhang, Ji, Hua, Ying, Jernberg, Cecilia, Xiong, Yanwen, French, Nigel, Löfgren, Sture, Hedenström, Ingela, Ambikan, Anoop, Mernelius, Sara, Matussek, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893091/
https://www.ncbi.nlm.nih.gov/pubmed/33613494
http://dx.doi.org/10.3389/fmicb.2021.627861
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author Bai, Xiangning
Zhang, Ji
Hua, Ying
Jernberg, Cecilia
Xiong, Yanwen
French, Nigel
Löfgren, Sture
Hedenström, Ingela
Ambikan, Anoop
Mernelius, Sara
Matussek, Andreas
author_facet Bai, Xiangning
Zhang, Ji
Hua, Ying
Jernberg, Cecilia
Xiong, Yanwen
French, Nigel
Löfgren, Sture
Hedenström, Ingela
Ambikan, Anoop
Mernelius, Sara
Matussek, Andreas
author_sort Bai, Xiangning
collection PubMed
description Shiga toxin-producing Escherichia coli (STEC) are important foodborne pathogens that can cause human infections ranging from asymptomatic carriage to bloody diarrhea (BD) and fatal hemolytic uremic syndrome (HUS). However, the molecular mechanism of STEC pathogenesis is not entirely known. Here, we demonstrated a large scale of molecular epidemiology and in-depth genomic study of clinical STEC isolates utilizing clinical and epidemiological data collected in Region Jönköping County, Sweden, over a 15-year period. Out of 184 STEC isolates recovered from distinct patients, 55 were from patients with BD, and 129 were from individuals with non-bloody stools (NBS). Five individuals developed HUS. Adults were more associated with BD. Serotypes O157:H7, O26:H11, O103:H2, O121:H19, and O104:H4 were more often associated with BD. The presence of Shiga toxin-encoding gene subtypes stx(2a), stx(2a) + stx(2c), and stx(1a) + stx(2c) was associated with BD, while stx(1)(a) was associated with milder disease. Multiplex virulence and accessory genes were correlated with BD; these genes encode toxins, adhesion, autotransporters, invasion, and secretion system. A number of antimicrobial resistance (AMR) genes, such as aminoglycoside, aminocoumarin, macrolide, and fluoroquinolone resistance genes, were prevalent among clinical STEC isolates. Whole-genome phylogeny revealed that O157 and non-O157 STEC isolates evolved from distinct lineages with a few exceptions. Isolates from BD showed more tendency to cluster closely. In conclusion, this study unravels molecular trait of clinical STEC strains and identifies genetic factors associated with severe clinical outcomes, which could contribute to management of STEC infections and disease progression if confirmed by further functional validation.
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spelling pubmed-78930912021-02-20 Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden Bai, Xiangning Zhang, Ji Hua, Ying Jernberg, Cecilia Xiong, Yanwen French, Nigel Löfgren, Sture Hedenström, Ingela Ambikan, Anoop Mernelius, Sara Matussek, Andreas Front Microbiol Microbiology Shiga toxin-producing Escherichia coli (STEC) are important foodborne pathogens that can cause human infections ranging from asymptomatic carriage to bloody diarrhea (BD) and fatal hemolytic uremic syndrome (HUS). However, the molecular mechanism of STEC pathogenesis is not entirely known. Here, we demonstrated a large scale of molecular epidemiology and in-depth genomic study of clinical STEC isolates utilizing clinical and epidemiological data collected in Region Jönköping County, Sweden, over a 15-year period. Out of 184 STEC isolates recovered from distinct patients, 55 were from patients with BD, and 129 were from individuals with non-bloody stools (NBS). Five individuals developed HUS. Adults were more associated with BD. Serotypes O157:H7, O26:H11, O103:H2, O121:H19, and O104:H4 were more often associated with BD. The presence of Shiga toxin-encoding gene subtypes stx(2a), stx(2a) + stx(2c), and stx(1a) + stx(2c) was associated with BD, while stx(1)(a) was associated with milder disease. Multiplex virulence and accessory genes were correlated with BD; these genes encode toxins, adhesion, autotransporters, invasion, and secretion system. A number of antimicrobial resistance (AMR) genes, such as aminoglycoside, aminocoumarin, macrolide, and fluoroquinolone resistance genes, were prevalent among clinical STEC isolates. Whole-genome phylogeny revealed that O157 and non-O157 STEC isolates evolved from distinct lineages with a few exceptions. Isolates from BD showed more tendency to cluster closely. In conclusion, this study unravels molecular trait of clinical STEC strains and identifies genetic factors associated with severe clinical outcomes, which could contribute to management of STEC infections and disease progression if confirmed by further functional validation. Frontiers Media S.A. 2021-02-05 /pmc/articles/PMC7893091/ /pubmed/33613494 http://dx.doi.org/10.3389/fmicb.2021.627861 Text en Copyright © 2021 Bai, Zhang, Hua, Jernberg, Xiong, French, Löfgren, Hedenström, Ambikan, Mernelius and Matussek. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bai, Xiangning
Zhang, Ji
Hua, Ying
Jernberg, Cecilia
Xiong, Yanwen
French, Nigel
Löfgren, Sture
Hedenström, Ingela
Ambikan, Anoop
Mernelius, Sara
Matussek, Andreas
Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden
title Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden
title_full Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden
title_fullStr Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden
title_full_unstemmed Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden
title_short Genomic Insights Into Clinical Shiga Toxin-Producing Escherichia coli Strains: A 15-Year Period Survey in Jönköping, Sweden
title_sort genomic insights into clinical shiga toxin-producing escherichia coli strains: a 15-year period survey in jönköping, sweden
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893091/
https://www.ncbi.nlm.nih.gov/pubmed/33613494
http://dx.doi.org/10.3389/fmicb.2021.627861
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