Cargando…

Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells

The components of ginger root (Zingiber officinale Roscoe) are widely used for various medicinal purposes. Several bioactive compounds have been identified in ginger, including 6-, 8- and 10-gingerols, and 6-shogaol, which are agonists of the thermo-sensors transient receptor potential (TRP) cation...

Descripción completa

Detalles Bibliográficos
Autores principales: Sugimoto, Naotoshi, Katakura, Masanori, Matsuzaki, Kentaro, Miyamoto, Mayumi, Sumiyoshi, Eri, Wada, Taizo, Yachie, Akihiro, Nakamura, Hiroyuki, Shido, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893714/
https://www.ncbi.nlm.nih.gov/pubmed/33537806
http://dx.doi.org/10.3892/mmr.2021.11889
_version_ 1783653101945749504
author Sugimoto, Naotoshi
Katakura, Masanori
Matsuzaki, Kentaro
Miyamoto, Mayumi
Sumiyoshi, Eri
Wada, Taizo
Yachie, Akihiro
Nakamura, Hiroyuki
Shido, Osamu
author_facet Sugimoto, Naotoshi
Katakura, Masanori
Matsuzaki, Kentaro
Miyamoto, Mayumi
Sumiyoshi, Eri
Wada, Taizo
Yachie, Akihiro
Nakamura, Hiroyuki
Shido, Osamu
author_sort Sugimoto, Naotoshi
collection PubMed
description The components of ginger root (Zingiber officinale Roscoe) are widely used for various medicinal purposes. Several bioactive compounds have been identified in ginger, including 6-, 8- and 10-gingerols, and 6-shogaol, which are agonists of the thermo-sensors transient receptor potential (TRP) cation channel subfamily V member 1 and TRP ankyrin 1. Our previous study demonstrated that ginger powder may affect human metabolism in vivo. However, the effects of the bioactive compounds of ginger on cells have not been completely elucidated. The present study investigated whether ginger powder extracts could modify cell functions in mouse fibroblast cells. The active components of ginger powder extracts were characterized using high-performance liquid chromatography. The activation of protein kinases, actin assembly, cell migration, expression levels of heat shock proteins (HSPs) and cell viability after heat shock were analyzed in NIH3T3 mouse fibroblast cells. Subsequently, 6-, 8-, 10- and 12-gingerols, as well as 6-, 8- and 10-shogaols, were detected in ginger powder extracts. The levels of phosphorylated Akt, mTOR, ERK and p38 MAPK increased after a 10-min stimulation with ginger powder extracts. In addition, HSP expression levels, lamellipodia formation occurring at cell edges, cell migration and tolerance against heat shock were facilitated following ginger powder extract stimulation. These results suggest that ginger modified cell functions, including actin assembly and heat tolerance, in vitro.
format Online
Article
Text
id pubmed-7893714
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-78937142021-03-08 Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells Sugimoto, Naotoshi Katakura, Masanori Matsuzaki, Kentaro Miyamoto, Mayumi Sumiyoshi, Eri Wada, Taizo Yachie, Akihiro Nakamura, Hiroyuki Shido, Osamu Mol Med Rep Articles The components of ginger root (Zingiber officinale Roscoe) are widely used for various medicinal purposes. Several bioactive compounds have been identified in ginger, including 6-, 8- and 10-gingerols, and 6-shogaol, which are agonists of the thermo-sensors transient receptor potential (TRP) cation channel subfamily V member 1 and TRP ankyrin 1. Our previous study demonstrated that ginger powder may affect human metabolism in vivo. However, the effects of the bioactive compounds of ginger on cells have not been completely elucidated. The present study investigated whether ginger powder extracts could modify cell functions in mouse fibroblast cells. The active components of ginger powder extracts were characterized using high-performance liquid chromatography. The activation of protein kinases, actin assembly, cell migration, expression levels of heat shock proteins (HSPs) and cell viability after heat shock were analyzed in NIH3T3 mouse fibroblast cells. Subsequently, 6-, 8-, 10- and 12-gingerols, as well as 6-, 8- and 10-shogaols, were detected in ginger powder extracts. The levels of phosphorylated Akt, mTOR, ERK and p38 MAPK increased after a 10-min stimulation with ginger powder extracts. In addition, HSP expression levels, lamellipodia formation occurring at cell edges, cell migration and tolerance against heat shock were facilitated following ginger powder extract stimulation. These results suggest that ginger modified cell functions, including actin assembly and heat tolerance, in vitro. D.A. Spandidos 2021-04 2021-02-02 /pmc/articles/PMC7893714/ /pubmed/33537806 http://dx.doi.org/10.3892/mmr.2021.11889 Text en Copyright: © Sugimoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Sugimoto, Naotoshi
Katakura, Masanori
Matsuzaki, Kentaro
Miyamoto, Mayumi
Sumiyoshi, Eri
Wada, Taizo
Yachie, Akihiro
Nakamura, Hiroyuki
Shido, Osamu
Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells
title Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells
title_full Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells
title_fullStr Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells
title_full_unstemmed Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells
title_short Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells
title_sort ginger facilitates cell migration and heat tolerance in mouse fibroblast cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893714/
https://www.ncbi.nlm.nih.gov/pubmed/33537806
http://dx.doi.org/10.3892/mmr.2021.11889
work_keys_str_mv AT sugimotonaotoshi gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT katakuramasanori gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT matsuzakikentaro gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT miyamotomayumi gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT sumiyoshieri gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT wadataizo gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT yachieakihiro gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT nakamurahiroyuki gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells
AT shidoosamu gingerfacilitatescellmigrationandheattoleranceinmousefibroblastcells